杨芳;万丽;胡一晨;吕维;张艺;
YANG Fang1,WAN Li1*,HU Yichen1,LV Wei1,ZHANG Yi2(1.College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China; 2.College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

• 1:成都中医药大学药学院教育部中药材标准化重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地
• 2:成都中医药大学民族医药学院

摘要(Abstract)：

通过建立川白芷中欧前胡素、异欧前胡素、氧化前胡素的"一测多评"含量测定方法,实验只用一个对照品同步测定川白芷中的3个成分。以欧前胡素对照品为内标物,在线性范围内,采用HPLC-DAD建立其与异欧前胡素、氧化前胡素的相对校正因子,并用该校正因子进行异欧前胡素、氧化前胡素的含量计算,实现"一测多评";同时采用外标法测定药材中这3种香豆素的含量,并比较计算值与实测值的差异。结果 20批药材中3种香豆素含量的计算值与实测值无显著差异(RSD<5%)。研究结果表明"一测多评"法控制川白芷药材的质量是可行、准确的。
Objective: To develop a quantitative analysis of multi-components by single marker(QAMS) method for simultaneous determination of three coumarins in Angelica dahurica var.formosana,by using one standard substance.Method: With imperatorin as internal reference standard,and 2 relative correction factors(RCF) to imperatorin were calculated within certain ranges.Their contents in 20 batches of samples,collected from different areas,were determined by both external standard method and QAMS.The method was evaluated by comparison of the quantitative results between the two methods.Result: No significant differences were found in the quantitative results of three coumarins in 20 batches of A.dahurica var.formosana determined by the two methods(RSD<5%).Conclusion: QAMS is feasible and suitable for quality control of A.dahurica var.formosana.

关键词(KeyWords)： 一测多评;校正因子;川白芷;质量控制
quantitative analysis of multi-components by single marker(QAMS);relative correction factor(RCF);Angelica dahurica var.formosana;quality control

Abstract:

Keywords:

基金项目(Foundation): 国家“十一五”科技支撑计划专项(2007BAI40B05,2007BAI40B03)

作者(Author): 杨芳;万丽;胡一晨;吕维;张艺;
YANG Fang1,WAN Li1*,HU Yichen1,LV Wei1,ZHANG Yi2(1.College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China; 2.College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

Email:

DOI:

参考文献(References)：

• [1]中国药典.一部[S].2010:97.
• [2]何开家,张涵庆.白芷化学成分及其药理研究进展[J].现代中药研究与实践,2008,22(3):59.
• [3]张富强,聂红,韦艺,等.白芷的化学与药理研究进展[J].南京中医药大学学报:自然科学版,2002(3):190.
• [4]李宏宇,戴跃进,谢成科,等.川白芷中香豆素类成分的反相高效液相色谱分析[J].华西药学杂志,1990,5(4):231.
• [5]王梦月,贾敏如,马逾英,等.不同入药部分及不同加工方法对白芷香豆素类成分含量的影响[J].中药材,2004,27(11):826.
• [6]Gao X Y,Jiang Y,Lu J Q,et al.One single standard substancefor the determination of multiple anthraquinone derivatives in rhu-barb using high-performance liquid chromatography-diode array de-tection[J].J Chromatogr A,2009,1216(11):2118.
• [7]王智民,钱忠直,张启伟,等.一测多评法建立的技术指南[J].中国中药杂志,2011,36(6):657.