中国中药杂志

2002, (10)

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高效液相色谱法测定地锦草中槲皮素的含量
Determination of Quercetin in the Herba Euphorbiae Humifusae

邢俊波,水彩虹,刘云
XING Jun-bo, SHUI Cai-hong, LIU Yun (Institute for Drug and Instrument Control Health Dept GLD of PLA, Beijing 100071, China)

摘要(Abstract):

目的 :建立地锦草中槲皮素高效液相色谱测定方法。方法 :色谱柱Kromasil C18(4.6mm× 2 5 0mm ,5μm) ,流动相为乙腈 0 .4 %磷酸 (40∶6 0 ) ,检测波长 370nm ,流速 1.0mL·min-1。结果 :槲皮素在 0 .0 36 4~ 0 .12 74μg线性关系良好 (r =0 .9999) ,回收率 10 0 .7% ,RSD 2 .5 %。 结论 :为地锦草及其制剂提供了分析方法 ,该法简便 ,结果可靠。
Objective: To develop an HPLC method for determining quercetin in Herba Euphorbiae Humifusae. Method: ODS column was used with acetonitrile: 0.4% phosphoric acid (40∶60) as mobile phase. The detection wavelength was at 370 nm and the flow rate was 1.0 mL·min -1. Result: The linearity was obtained over the range of 0.0364~0.1274 μg (r=0.9999). The average recovery was 100.71% with relative standard deviation of 2.45%. Conclusion: This method can be used for quality control of Herba Euphorbiae Humifusae. It is simple, rapid and reliable.

关键词(KeyWords): 地锦草;槲皮素;含量测定;高效液相色谱法
Herba Euphorbiae Humifusae; quercetin; quantitative determination; HPLC

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作者(Author): 邢俊波,水彩虹,刘云
XING Jun-bo, SHUI Cai-hong, LIU Yun (Institute for Drug and Instrument Control Health Dept GLD of PLA, Beijing 100071, China)

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