中国中药杂志

2008, (04) 432-435

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伪人参皂苷GQ的排泄试验研究
Study on excretion of pseudo-ginsenoside GQ

赵春芳;刘金平;赵岩;李平亚;
ZHAO Chun-fang1,LIU Jin-ping2,ZHAO Yan2,LI Ping-ya2(1.Pharmaceutical Academy of Jilin University,Changchun 130021,China;2.Institute of Frontier Medical Science of Jilin University,Changchun 130021, China)

摘要(Abstract):

目的:研究大鼠舌下静脉给药伪人参皂苷GQ后,其在胆汁、粪和尿中的排泄情况。方法:采用高效液相-蒸发光散射色谱(HPLC-ELSD)法测定大鼠胆汁、粪和尿中伪人参皂苷GQ,Diamonsil C18色谱柱(4.6 mm×250mm,5μm),以甲醇-水(24∶7)为流动相,流速1.0 mL.min-1,检测温度为50℃,灵敏度为10,以氮气为载气,压力为303 975 Pa。结果:HPLC-ELSD测定方法的标准曲线线性关系、样品回收率和日内、日间精密度均符合要求。伪人参皂苷GQ大鼠舌下静脉给药后,主要以胆汁排泄为主,占总药量的41.60%;其次为粪排泄,占总药量的9.97%;尿液中仅检出少量伪人参皂苷GQ。结论:大鼠胆汁、粪和尿中主要以伪人参皂苷GQ原形药物排泄。
Objective: To determine the pseudo-ginsenoside GQ(PGQ) in rat bile,feces and urine,and to study on the excretion of pseudo-ginsenoside GQ.Method: Reverse phase high-performance liquid chromatography(RP-HPLC) method with an evaporative light-scattering detector(ELSD) was performed on DiamonsilTM C18 column(4.6 mm×250 mm,5 μm),and the mobile phase was consisted of methanol-water(24∶7) with flow rate of 1.0 mL·min-1.ELSD parameters were set as follows: nitrogen gas pressure 3.0 bar,drift tube temperature 50 ℃.Result: The method fulfilled all the standard requirements of precision,accuracy and linearity.The main way of excretion of PGQ in rat administrated through sublingual vein was at the bile.The bile excretion ratio of PGQ was 41.60%,and feces excretion ratio was 9.97%.Only trace amount of PGQ was excreted in urine.Conclusion: Almost all unchanged PGQ was excreted in bile,feces and urine.

关键词(KeyWords): 伪人参皂苷GQ;蒸发光散射检测法;药物排泄
pseudo-ginsenoside GQ;ELSD;excretion

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作者(Author): 赵春芳;刘金平;赵岩;李平亚;
ZHAO Chun-fang1,LIU Jin-ping2,ZHAO Yan2,LI Ping-ya2(1.Pharmaceutical Academy of Jilin University,Changchun 130021,China;2.Institute of Frontier Medical Science of Jilin University,Changchun 130021, China)

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