中国中药杂志

2010, v.35(16) 2072-2074

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

HPLC测定王不留行中王不留行黄酮苷的含量
Determination of vaccarin in Vaccariae Semen by HPLC

孟贺;陈玉平;秦文杰;唐晓晶;叶祖光;
MENG He,CHEN Yuping,QIN Wenjie,TANG Xiaojing,YE Zuguang (National Engineering Research Center for Research and Development of Traditional Chinese Medicine Multi-ingredient Drugs,Beijing 100075,China)

摘要(Abstract):

目的:建立王不留行中王不留行黄酮苷的含量测定方法。方法:运用高效液相色谱法,Alltima-C18(4.6 mm×250mm,5μm)色谱柱,以甲醇-0.3%磷酸溶液为流动相,梯度洗脱,流速1.0 mL.min-1,检测波长280 nm。结果:王不留行黄酮苷在0.102~1.539μg线性关系良好(R2=0.999 7),平均加样回收率为100.4%,RSD 0.81%。10批样品含量测定表明,王不留行黄酮苷质量分数为0.46%~0.57%。结论:本法简便、准确、重复性好、专属性强,为王不留行药材质量控制提供了方法。
To establish a HPLC method for the determination of vaccarin in Vaccariae Semen.Analysis was carried out on an Alltima-C18 column(4.6 mm×250 mm,5 μm) eluted with methanol-0.3% phosphoric acid as mobile phase in gradient elution.The flow rate was 1.0 mL·min-1 and detected wavelength was set at 280 nm.The peak areas and injection ammounts of vaccarin showed a good linear relationship in the range of 0.102-1.539 μg,R2=0.999 7.The average recovery was 100.4%,RSD was 0.81%.The results of the assay of 10 samples showed that the contents of vaccarin varied in the range of 0.46%-0.57%.The method is simple,accurate,reproducible and specific.It can be used for the quality control of Vaccariae Semen.

关键词(KeyWords): 王不留行;王不留行黄酮苷;高效液相色谱法;质量标准
Vaccariae Semen;vaccarin;HPLC;quality standard

Abstract:

Keywords:

基金项目(Foundation): 《中国药典》2010版一部标准研究(YD-158)

作者(Author): 孟贺;陈玉平;秦文杰;唐晓晶;叶祖光;
MENG He,CHEN Yuping,QIN Wenjie,TANG Xiaojing,YE Zuguang (National Engineering Research Center for Research and Development of Traditional Chinese Medicine Multi-ingredient Drugs,Beijing 100075,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享