中国中药杂志

2021, v.46(06) 1410-1416

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基于指纹图谱和多成分含量测定的白芍与炒白芍标准汤剂质量差异性研究
Research on quality difference of standard decoction of raw and fried Paeoniae Radix Alba based on fingerprint and multicomponent determination

干丽;严玉晶;丁青;洪婉敏;纪玉华;杨晓东;刘燎原;魏梅;
GAN Li;YAN Yu-jing;DING Qing;HONG Wan-min;JI Yu-hua;YANG Xiao-dong;LIU Liao-yuan;WEI Mei;Guangdong Province Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yifang Pharmaceutical Co.,Ltd.;

摘要(Abstract):

该研究通过测定白芍与炒白芍标准汤剂的出膏率、多成分含量、指纹图谱,比较二者质量的差异性。建立17批白芍炮制前后标准汤剂的UPLC指纹图谱,同时测定没食子酸、儿茶素、芍药内酯苷、芍药苷、苯甲酰芍药苷的含量,对白芍与炒白芍标准汤剂峰面积进行独立样本t检验和正交偏最小二乘判别分析。白芍与炒白芍标准汤剂的出膏率无明显差异,炮制后标准汤剂中芍药内酯苷的平均含量增加了0.26%,没食子酸、儿茶素、芍药苷、苯甲酰芍药苷的平均含量分别降低了13.04%,27.94%,10.30%,18.79%。白芍标准汤剂指纹图谱标定了14个共有峰,炒白芍标准汤剂指纹图谱标定了16个共有峰,峰1与峰3为炮制后新增的色谱峰,其中峰3为5-羟甲基糠醛;独立样本t检验和正交偏最小二乘判别分析结果表明峰1、峰3、峰11、峰15是区分白芍与炒白芍标准汤剂关键的化合物,研究表明该方法可用于白芍与炒白芍标准汤剂、颗粒剂等制剂过程中各工序的量值传递和质量控制研究,同时为白芍生品与炮制品相关制剂的鉴别提供参考。
The extract rates, multicomponent content and fingerprint were determined in this study to investigate the quality diffe-rence between standard decoction of raw Paeoniae Radix Alba and fried Paeoniae Radix Alba. UPLC fingerprint was established for 17 batches of standard decoction of raw and fried Paeoniae Radix Alba, and the contents of gallic acid, catechin, albiflorin, paeoniflorin and benzoyl paeoniflorin were determined. The peak areas of standard decoction were analyzed by the independent t-test and orthogonal partial least squares discriminant analysis. There was no significant difference in extract rates between the standard decoction of raw and fried Paeoniae Radix Alba. After fried processing, the content of albiflorin increased by 0.26%, while the contents of gallic acid, catechin, paeoniflorin and benzoyl paeoniflorin decreased by 13.04%, 27.97%, 10.30% and 18.79% respectively. There were 14 common peaks in the fingerprint of standard decoction of raw Paeoniae Radix Alba, and 16 common peaks in the fried Paeoniae Radix Alba. Peak 1 and peak 3 were new ones after processing, among which the peak 3 was 5-hydroxymethylfurfural. The results showed that peak 1, peak 3, peak 11 and peak 15 were the key compounds to distinguish standard decoction of raw and fried Paeoniae Radix Alba. In conclusion, this method is stable and can be used for the study of quantity transfer and quality control in the preparation process of standard decoction, granules and other dosage forms for raw and fried Paeoniae Radix Alba, providing reference for the identification of raw and fried Paeoniae Radix Alba and related preparations.

关键词(KeyWords): 白芍;炒白芍;标准汤剂;指纹图谱;独立样本t检验;正交偏最小二乘判别分析;质量控制
Paeoniae Radix Alba;fried Paeoniae Radix Alba;standard decoction;fingerprint;independent t-test;orthogonal partial least squares discriminant analysis;quality control

Abstract:

Keywords:

基金项目(Foundation): 广东省中药配方颗粒企业重点实验室项目(2018B030323004)

作者(Author): 干丽;严玉晶;丁青;洪婉敏;纪玉华;杨晓东;刘燎原;魏梅;
GAN Li;YAN Yu-jing;DING Qing;HONG Wan-min;JI Yu-hua;YANG Xiao-dong;LIU Liao-yuan;WEI Mei;Guangdong Province Key Laboratory of Traditional Chinese Medicine Formula Granule,Guangdong Yifang Pharmaceutical Co.,Ltd.;

Email:

DOI: 10.19540/j.cnki.cjcmm.20201130.301

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