中国中药杂志

2014, v.39(07) 1185-1189

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

藏药翼首草UFLC指纹图谱研究
UFLC-PDA fingerprint of Tibetan medicine Pterocephalus hookeir

高燕;李文婕;李聪颖;范刚;张艺;
GAO Yan;LI Wen-jie;LI Cong-ying;FANG Gang;ZHANG Yi;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;

摘要(Abstract):

建立不同产地藏药翼首草的UFLC指纹图谱分析方法。实验采用UFLC-PDA检测法,使用Agilent Proshell 120SB-C18色谱柱(4.6 mm×100 mm,2.7μm),以乙腈-0.2%磷酸水溶液为流动相梯度洗脱,流速1.0 mL·min-1,柱温为30℃,检测波长238 nm。建立了21个批次翼首草药材的共有图谱,提取了15个色谱峰作为指纹图谱共有峰,结合对照品对其中的5个色谱峰进行了指认,分别为绿原酸、马钱苷、獐芽菜苷、吴茱萸苷、大花双参苷A;21批药材中第13,18,20批次与对照指纹图谱比较相似度小于0.9,其他批次的相似度均大于0.9。该研究首次采用UFLC-PDA建立藏药翼首草的指纹图谱,该方法具有良好的精密度、稳定性和重复性,为翼首草质量控制和评价提供依据。
This study was aimed to establish an UFLC fingerprint of Tibetan medicine Pterocephalus hookeir samples from different habitats. UFLC-PDA was adopted to analyse 21 batches of P. hookeir samples from different habitats. The chromatographic condition was as follow: Agilent proshell 120 SB-C18column( 4. 6 mm × 100 mm,2. 7 μm) eluted with the mobile phases of acetonitrile and 0. 2% phosphoric acid water in gradient mode. The flow rate was 1. 0 mL·min-1,and the detection wavelength was set at 238 nm. The fingerprints of 21 batches P. hookeir were carried out by similarity comparation,and 15 chromatographic peaks were extracted as the common peaks of fingerprint,of which 5 peaks were identified as chlorogenic acid,loganin,sweroside,sylvestroside Ⅲ,triplostoside A. The similarity degrees of 18 batchs of samples were above 0. 9,and the other 3 batchs of samples were below 0. 9. This is the first established fingerprint of P. hookeir by using UFLC-PDA. This method has good precision,stability and repeatability that it could provide basis for quality control and evaluation of P. hookeir.

关键词(KeyWords): 藏药;翼首草;UFLC-PDA;指纹图谱
Tibetan medicinal material;Pterocephalus hookeir;UFLC-PDA;chromatographic fingerprint

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81274193);; 四川省教育厅四川省省属高校科研创新团队建设计划项目(11TD004)

作者(Author): 高燕;李文婕;李聪颖;范刚;张艺;
GAO Yan;LI Wen-jie;LI Cong-ying;FANG Gang;ZHANG Yi;College of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine;

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享