中国中药杂志

2020, v.45(02) 418-424

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基于HPLC-QqQ-MS研究肠道菌群对藏族药小檗皮5种入血成分的影响
Effects of gut microbiota on five absorbed components of Berberis kansuensis in rat serum by HPLC-QqQ-MS

杜欢;徐鑫梅;徐僮;李琪;赵程成;易欢;范刚;
DU Huan;XU Xin-mei;XU Tong;LI Qi;ZHAO Cheng-cheng;YI Huan;FAN Gang;College of Pharmacy, Chengdu University of Traditional Chinese Medicine;College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine;

摘要(Abstract):

为阐明小檗皮中生物碱类成分在生物体内吸收代谢情况,该研究采用HPLC-QqQ-MS技术,以乙腈-0.1%甲酸水为流动相,梯度洗脱,并在多反应监测模式下进行入血成分的定性及定量分析。此外,为探讨肠道菌群对小檗皮生物碱类成分体内代谢的影响,建立糖尿病大鼠与伪无菌糖尿病大鼠模型,结合偏最小二乘法判别分析和单因素方差分析寻找不同组间入血成分的含量差异。该研究首次建立了小檗皮5种入血成分的定量分析方法,发现不同组间小檗皮入血成分存在差异。通过正常组与糖尿病模型组比较,发现在健康和病理2种不同状态下,小檗皮中小檗碱、木兰花碱的吸收代谢能力不同;并且发现伪无菌糖尿病大鼠血清中小檗碱、木兰花碱、药根碱的含量显著低于糖尿病大鼠。研究结果揭示了肠道菌群在小檗皮生物碱类成分体内代谢过程中的重要作用,可为小檗皮的药效物质基础研究提供参考。
To elucidate the absorption and metabolism of alkaloids in Berberis kansuensis in vivo, a high performance liquid chromatography-triple quadrupole mass spectrometry(HPLC-QqQ-MS) method was developed to qualitatively and quantitatively analyze the absorption components in rat serum in multiple-reaction monitoring mode. The mobile phase consisted of 0.1% formic acid and acetonitrile with a gradient elution mode. In addition, to investigate the effects of gut microbiota on five absorbed components of B. kansuensis in rat serum, diabetic rat and pseudo germ-free diabetic rat models were established, and partial least squares discriminant analysis and One-way ANOVA were used to study the content differences of five components among different groups. In this study, a HPLC-QqQ-MS method for quantitative analysis of five components in rat serum after oral administration of B. kansuensis was established for the first time. It was found that there were differences in the five constituents in rat serum between different groups. By comparing the normal group with the diabetic model group, we found that the absorption and metabolism capacities of berberine and magnoflorine were different under the health and pathological conditions. It was also found that the serum levels of berberine, magnoflorine and jatrorrhizine in pseudo germ-free diabetic rats were significantly lower than those in diabetic rats, indicating that gut microbiota plays an important role in the metabolism of alkaloids of B. kansuensis in vivo. These results provide a good reference for clarifying the active ingredients of B. kansuensis in the treatment of diabetes.

关键词(KeyWords): 小檗皮;糖尿病;生物碱;HPLC-QqQ-MS;肠道菌群
Berberis kansuensis;diabetes;alkaloids;HPLC-QqQ-MS;gut microbiota

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81874370,81303310);; 成都市科技局项目(2018-YFYF-00053-SN)

作者(Author): 杜欢;徐鑫梅;徐僮;李琪;赵程成;易欢;范刚;
DU Huan;XU Xin-mei;XU Tong;LI Qi;ZHAO Cheng-cheng;YI Huan;FAN Gang;College of Pharmacy, Chengdu University of Traditional Chinese Medicine;College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine;

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DOI: 10.19540/j.cnki.cjcmm.20190830.203

参考文献(References):

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