李龙飞;马增春;王宇光;汤响林;谭洪玲;肖成荣;高月;

• 1:军事医学科学院放射与辐射医学研究所

摘要(Abstract)：

该研究以H9c2细胞为研究对象,采用阿霉素诱导心肌细胞自噬活化,探讨人参皂苷Rb_1对阿霉素诱导心肌细胞自噬的作用。应用CCK-8法、透射电镜观察、荧光染色观察、Western blot等方法分别检测药物作用后H9c2细胞的增殖以及自噬变化。结果显示,阿霉素引起H9c2细胞活力下降,导致H9c2细胞的自噬相关结构增加、自噬标志性蛋白LC3由LC3-I转变为LC3-Ⅱ增加及p62蛋白表达降低;人参皂苷Rb_1可以减弱阿霉素引起的心肌细胞活力下降并抑制阿霉素诱导的自噬相关结构增加、LC3-I转变为LC3-Ⅱ的增加以及p62蛋白表达的降低。以上结果提示,阿霉素可引起H9c2细胞死亡并诱导细胞自噬的活化,而人参皂苷Rb_1对阿霉素诱导心肌细胞死亡具有保护作用,这种作用可能是通过调节自噬产生的。

关键词(KeyWords)： 人参皂苷Rb1;阿霉素;自噬;H9c2细胞

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81630102,81673633)

作者(Author): 李龙飞;马增春;王宇光;汤响林;谭洪玲;肖成荣;高月;

Email:

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