中国中药杂志

2015, v.40(11) 2077-2083

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厚朴MVA途径相关基因鉴定及生物信息学分析
Identification and bioinformatics analysis of genes associated with MVA pathway in Magnolia officinalis

查良平;袁媛;黄璐琦;余淑琳;
ZHA Liang-ping;YUAN Yuan;HUANG Lu-qi;YU Shu-lin;College of Pharmacy,Chengdu University of Traditional Chinese Medicine;State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

摘要(Abstract):

甲羟戊酸(MVA)途径是萜类化合物生物合成的重要途径,该研究在厚朴转录组测序基础上,利用生物信息学方法对MVA途径中相关基因Mo ACOT,Mo HMGS,Mo HMGR,Mo MK进行全面的生物信息学分析。结果表明,Mo ACOT和Mo MK为稳定的亲水蛋白,而Mo HMGS,Mo HMGR为不稳定的亲水蛋白;二级结构均为混合型结构的蛋白质,α-螺旋是所有基因多肽链中大量的结构元件;利用同源建模法对三级空间结构进行了预测;跨膜域分析显示Mo ACOT,Mo HMGS,Mo MK无跨膜区,MoHMGR具有2个跨膜区,分别为39~61 aa和82~104 aa;系统进化树分析表明Mo ACOT,Mo HMGS,Mo HMGR,Mo MK均与被子植物或双子叶植物遗传距离较近,符合遗传进化规律;基因表达水平显示Mo ACOT,Mo HMGS,Mo HMGR,Mo MK表达水平在厚朴与凹叶厚朴间均无显著差异。研究结果为解析厚朴萜类生物合成MVA途径的分子机制提供理论依据。
Methyl valerate(MVA) pathway is one of the important ways for synthesis of terpenoids. This study was based on data of the transcriptome sequencing of Magnolia officinalis,the associated genes Mo ACOT,Mo HMGS,Mo HMGR,Mo MK in methyl valerate(MVA) pathway,were completed in detail by using bioinformatics methods. The results of analysis showed that Mo ACOT and Mo MK were stable hydrophobic proteins,Mo HMGS and Mo HMGR were unstable hydrophobic protein. The secondary structures of all proteins were hybrid architecture,and alpha helical were the major motifs. There were no clear transmembrane domains in Mo ACOT,Mo HMGS and Mo MK,but two transmembrane domains were founded in Mo HMGR which were from 39-61 aa and 82-104 aa resepectively. The results of evolutionary relationship analysis showed that Mo ACOT,Mo HMGS,Mo HMGR and Mo MK had relative close relationship to angiosperm or dicotyledonous plants,and accorded with genetic evolution rule. From transcriptome data,transcripted level of Mo ACOT,MoHMGS,Mo HMGR,Mo MK in M. officinalis and M. officinalis var. biloba was not significantly different. The result provided theoretical reference for study on Methyl valerate( MVA) pathway of terpenoid of M. officinalis.

关键词(KeyWords): 厚朴;MVA途径;萜类;转录组;生物信息学
Magnolia officinalis;MVA pathway;terpenoids;transcriptome;bioinformatics

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基金项目(Foundation): 国家杰出青年科学基金项目(81325023);; 国家高技术研究发展计划(863)项目(SS2014AA022201)

作者(Author): 查良平;袁媛;黄璐琦;余淑琳;
ZHA Liang-ping;YUAN Yuan;HUANG Lu-qi;YU Shu-lin;College of Pharmacy,Chengdu University of Traditional Chinese Medicine;State Key Laboratory of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences;

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