查良平;袁媛;黄璐琦;余淑琳;

• 1:成都中医药大学药学院
• 2:中国中医科学院中药资源中心道地药材国家重点实验室培育基地

摘要(Abstract)：

甲羟戊酸(MVA)途径是萜类化合物生物合成的重要途径,该研究在厚朴转录组测序基础上,利用生物信息学方法对MVA途径中相关基因Mo ACOT,Mo HMGS,Mo HMGR,Mo MK进行全面的生物信息学分析。结果表明,Mo ACOT和Mo MK为稳定的亲水蛋白,而Mo HMGS,Mo HMGR为不稳定的亲水蛋白;二级结构均为混合型结构的蛋白质,α-螺旋是所有基因多肽链中大量的结构元件;利用同源建模法对三级空间结构进行了预测;跨膜域分析显示Mo ACOT,Mo HMGS,Mo MK无跨膜区,MoHMGR具有2个跨膜区,分别为39~61 aa和82~104 aa;系统进化树分析表明Mo ACOT,Mo HMGS,Mo HMGR,Mo MK均与被子植物或双子叶植物遗传距离较近,符合遗传进化规律;基因表达水平显示Mo ACOT,Mo HMGS,Mo HMGR,Mo MK表达水平在厚朴与凹叶厚朴间均无显著差异。研究结果为解析厚朴萜类生物合成MVA途径的分子机制提供理论依据。

关键词(KeyWords)： 厚朴;MVA途径;萜类;转录组;生物信息学

Abstract:

Keywords:

基金项目(Foundation): 国家杰出青年科学基金项目(81325023);; 国家高技术研究发展计划(863)项目(SS2014AA022201)

作者(Author): 查良平;袁媛;黄璐琦;余淑琳;

Email:

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