中国中药杂志

2009, v.34(13) 1695-1700

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31种黄酮、酚酸类化合物和10种中药清除DPPH能力考察
DPPH radical scavenging activities of 31 flavonoids and phenolic acids and 10 extracts of Chinese materia medica

袁亚男;陈承瑜;杨滨;小谷明;楠文代;
YUAN Yanan1,CHEN Chengyu1,YANG Bin1*,KUSU Fumiyo2,KOTANI Akira2 (1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.School of Pharmacy,Tokyo University of Pharmacy and Life Science,Tokyo 1432-1,Japan)

摘要(Abstract):

目的:考察中药中黄酮、酚酸类成分清除DPPH自由基活性及构效关系,中药乙醇提取物清除DPPH自由基的活性及其与黄酮、酚酸类成分总量之间的关系。方法:以中药中常见的31种黄酮、酚酸类化合物和10种中药为研究对象,以DPPH法对上述41个样品的抗氧化活性进行评价,以高效液相色谱-库仑阵列检测技术检测黄酮及酚酸类化合物的氧化电位,以Folin-C iocalteu法测定中药中黄酮和酚酸类成分的总量。结果:31种化合物清除DPPH自由基的能力有很大差别,其中以(-)-EGCg清除DPPH自由基的能力最强,其IC50值为6.7μmol.L-1;氧化电位值50 mV;提取物中,茶叶提取物中总黄酮和酚酸类成分的含量最高,为82.9%(以芦丁计),清除DPPH自由基能力最强,IC50值为0.012 g.L-1;红花醇提物总黄酮和酚酸类成分的含量最低,为7.01%(以芦丁计),其清除DPPH自由基能力最弱,其IC50值为1.3 g.L-1。结论:黄酮、酚酸类成分清除DPPH自由基的能力与化合物中酚羟基的取代个数和位置有关;中药清除DPPH自由基能力(Y)与总黄酮和酚酸类成分含量(X)之间存在相关性,Y=7.779X-0.48,r=0.929 5,即总黄酮和酚酸类成分含量高,清除DPPH自由基能力强。
Objective: To investigate DPPH radical scavenging activities of 31 flavonoids and phenolic acids and 10 extracts of Chinese materia medica.Method: The antioxidant activities of the above samples were evaluated by a DPPH method,the half-wave oxidation potentials(E1/2) of the 31 compounds were determined by an HPLC-CoulArray method,at the same time,phenolic contents of the the total compounds in the 10 extracts of Chinese materia medica were analyzed by Folin-Ciocalteu method.Result: The 31 compounds showed a 50% inhibition of DPPH radical in the concentration range of 6.7-3 500 μmol·L-1,in which(-)-EGCg demonstrated the strongest activity with the IC50 value of 6.7 μmol·L-1.The E1/2 of 31 compounds spanned a wide potential range of more than 0.6 V.Myricetin had the lowest E1/2 value(20 mV) whereas apigenin and vitexin-2″-O-rhamnoside had the highest E1/2 value(620 mV).Among the 10 herb extracts,having 82% phenolic acid,tea extract showed the strongest DPPH radical scavenging activity with the IC50 value of 0.011 7 mg·mL-1 whereas safflower demonstrated the weakest DPPH radical scavenging activity with the IC50 value of 1.250 mg·mL-1,in which only 7% phenolic acids was tested.Conclusion: The DPPH radical scavenging activities of the 31 compounds were founded to be related to their chemical structures,such as the number and position of hydroxyl groups.And a qualitative relationship was found between DPPH radical scavenging activities and E1/2 values of the 31 compounds,the lower the E1/2 values,the higher the DPPH radical scavenging activities.A quantitative relationship was obtained to describe the DPPH radical scavenging activity of the herb extracts: Y=7.779X-0.48,r=0.929 5,where Y stands for the concentration for 50% inhibition of DPPH radical,and X stands for the concentration of total phenolic compaunds,namely the extracts with higher content of flavonoids and phenolic acid exhibited the stronger DPPH radical scavenging activity.

关键词(KeyWords): 黄酮和酚酸类化合物;中药提取物;清除DPPH自由基能力;Folin-Ciocalteu法;总酚类含量;HPLC;氧化电位
flavonoids and phenolic acids;extracts of Chinese materia medica;DPPH radical scavenging activity;Folin-Ciocalteu method;total phenolic contents;HPLC;oxidation potential

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基金项目(Foundation): 国际科技合作项目(2006DFB31720)

作者(Author): 袁亚男;陈承瑜;杨滨;小谷明;楠文代;
YUAN Yanan1,CHEN Chengyu1,YANG Bin1*,KUSU Fumiyo2,KOTANI Akira2 (1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;2.School of Pharmacy,Tokyo University of Pharmacy and Life Science,Tokyo 1432-1,Japan)

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