中国中药杂志

2012, v.37(24) 3793-3798

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不同发育阶段对黄芩生长及活性成分积累的影响
Effect of different developmental stage on plant growth and active compounds in Scutellaria baicalensis

胡国强;袁媛;伍翀;蒋超;汪周勇;林淑芳;吴志刚;
HU Guo-qiang1,2,YUAN Yuan1*,WU Chong1,JIANG Chao1,WANG Zhou-yong1,LIN Shu-fang1,WU Zhi-gang1(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China)

摘要(Abstract):

目的:分析黄芩生长发育及其活性成分积累的关键物候期。方法:将从山东莱芜收集的黄芩种子分别在北京房山和山东莱芜进行播种,选择萌芽期、展叶期、花果中期、花果末期、枯黄期收集黄芩的地上部和地下部,测定其主根长度、根鲜重、根径、茎长度。利用HPLC测定根中黄芩苷、黄芩素的含量;利用紫外分光光度法测定总黄酮含量。利用RT-PCR方法分析根组织中苯丙氨酸解氨酶(phenylalanine ammonia lyase,PAL1,PAL2,PAL3)、肉桂酸-4-羟基化酶(cinnamate-4-hydroxylase,C4H)、4-香豆酸辅酶A连接酶(4-coumaroyl-CoA ligase,4CL)、查尔酮合成酶(chaleone synthase,CHS)、葡萄糖苷酸酶(glucu-ronidase,GUS)和黄芩素7-O-葡萄糖醛酸基转移酶(UDP-glucuronate:baicalein 7-O-glucuronosyltransferase,UBGAT)的转录水平。结果:从幼苗期至展叶期,黄芩地上部生长较快,而根部的生长主要集中在花果中期至枯黄期。黄芩整个生长发育阶段总黄酮含量的变化不显著、黄芩苷含量逐渐上升、黄芩素含量逐渐下降。黄芩苷生物合成的关键酶基因PAL,4CL在枯黄期表达水平量最高;CHS转录水平则在花果中期-花果后期呈现显著上升的趋势,而后在枯黄期下降。结论:结合黄芩活性成分及关键酶基因的表达分析,展叶期和枯黄期可能是影响黄芩生长发育和活性成分含量的主要物候期。
Objective:To study the developmental phase on the growth and active compounds in Scutellaria baicalensis.Method: Seeds of wild plants were collected from Laiwu and sowed in Fangshan(Beijing) and Laiwu(Shandong).Samples of aerial and underground parts were collected in five growth periods of sprouts,seedlings,flowering,seed drop and withered periods respectively.The length of taproot,fresh weight of root,diameter of taproot and the length of stem were determined.The content of active compounds and total flavonoids were determined by HPLC and ultraviolet spectrophotometry respectively.The transcripted level of PAL1,PAL2,PAL3,C4H,4CL,CHS,GUS and UBGAT were analyzed with RT-PCR.Result: The results showed that the aerial part of S.baicalensis grew quickly before flowering stage,and the underground part grew mostly between the periods of flowering and withered.In the whole growing developmental periods,the content of total flavonoids was not changed significantly,the content of baicalin was increased gradually and the content of baicalein was decreased gradually.Expression level of PAL and 4CL was the highest in withered period,CHS was increased between flowering and seed drop and decreased in withered period.Conclusion: Seedlings and withered periods may be the key phase affecting the growth and active compounds in S.baicalensis.

关键词(KeyWords): 物候期;活性成分;基因表达
phenological phase;active compounds;growing development

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基金项目(Foundation): 北京市科技新星项目(2008B82);; 中国博士后科学基金项目

作者(Author): 胡国强;袁媛;伍翀;蒋超;汪周勇;林淑芳;吴志刚;
HU Guo-qiang1,2,YUAN Yuan1*,WU Chong1,JIANG Chao1,WANG Zhou-yong1,LIN Shu-fang1,WU Zhi-gang1(1.Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China; 2.School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100102,China)

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