中国中药杂志

2014, v.39(14) 2614-2618

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外源Ca~(2+)对高温胁迫下半夏光合参数及有效成分积累的影响
Effects of Ca~(2+) on photosynthetic parameters of Pinellia ternata and accumulations of active components in heat stress

杨卫星;黑刚刚;李姣姣;张红敏;李琳琳;吴能表;
YANG Wei-xing;HEI Gang-gang;LI Jiao-jiao;ZHANG Hong-min;LI Lin-lin;WU Neng-biao;School of Life Science,Southwest University,Key Laboratory of Eco-environments in Three Gorges Reservoir Region,Ministry of Education;

摘要(Abstract):

目的:研究叶喷施钙盐对高温胁迫下半夏的光合参数及3种有效成分积累的影响。方法:待半夏株高10 cm左右时,高温处理并喷施不同浓度的CaCl2溶液,18 d后测定半夏叶片叶绿素含量、光合作用参数及叶绿素荧光参数,并测定了块茎中鸟苷、腺苷及多糖的含量。结果:与对照相比,喷施Ca2+明显提高了叶绿素含量和叶绿素a/b;6 mmol·L-1Ca2+处理显著提高了叶片的净光合速率(Pn)、蒸腾作用(Tr)和气孔限制值(Ls),降低了胞间CO2浓度(Ci);随Ca2+浓度的升高,叶片最大光能转换效率(Fv/Fm)、实际光合效率(Yield)和光化学猝灭系数(qP)呈先升后降的趋势,初始荧光(Fo)及非光化学猝灭系数(NPQ)呈先降后升趋势;半夏块茎的干重及其中的鸟苷、多糖含量随Ca2+浓度的升高呈现先升后降趋势,腺苷含量则随之升高而升高;当Ca2+浓度为6 mmol·L-1时,块茎的干重、鸟苷及多糖含量最高。结论:叶面喷钙缓解了高温对半夏叶片光合作用的抑制以及对PSⅡ系统的损伤,从而提高半夏的产量。
Objective: To study the effect of exogenous Ca2+ on photosynthetic parameters of Pinellia ternate and accumulations of active components under high temperature stress. Method: The pigment contents of P. ternata leaves,photosynthesis parameters and chlorophyll fluorescence parameters of P. ternata leaves,the contents of guanosine,adenosine and polysaccharide in P. ternata tubers were measured based on different concentrations of exogenous Ca2+in heat stress when the plant height of P. ternata was around 10 cm. Result: The contents of total chlorophyll and ratio of chlorophyll a /b were relatively higher by spaying Ca2+. Compared with the control,spaying 6 mmol·L-1Ca2+ significantly enhanced the net photosynthetic rate( Pn),transpiration( Tr) and stomatal limitation( Ls),but reduced intercellular CO2 concentration( Ci) in P. ternata leaves. With the increase of Ca2+concentration,maximal PSⅡefficiency( Fv/Fm),actual photosynthetic efficiency( Yield) and photochemical quenching coefficient( qP) initially increased and then decreased,however,minimal fluorescence( Fo) and non-photochemical quenching coefficient( NPQ) went down first and then went up. The contents of guanosine and polysaccharide and dry weight of P. ternata tubers showed a tendency of increase after decrease,and the content of adenosine increased with the increase of Ca2+ concentration. The content of guanosine and polysaccharide in P. ternata tubers and its dry weight reached maximum when spaying 6 mmol·L-1Ca2+. Conclusion: With the treatment of calcium ion,the inhibition of photosynthesis and the damage of PSⅡ system were relieved in heat stress,which increased the production of P. ternata tubers.

关键词(KeyWords): 半夏;叶绿素含量;光合作用;叶绿素荧光;有效成分
Pinellia ternate;the content of chlorophyll;photosynthesis;chlorophyll fluorescence;active components

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基金项目(Foundation): 国家自然科学基金项目(30500041);; 重庆市科技攻关项目(cstc2012gg-yyjs80013)

作者(Author): 杨卫星;黑刚刚;李姣姣;张红敏;李琳琳;吴能表;
YANG Wei-xing;HEI Gang-gang;LI Jiao-jiao;ZHANG Hong-min;LI Lin-lin;WU Neng-biao;School of Life Science,Southwest University,Key Laboratory of Eco-environments in Three Gorges Reservoir Region,Ministry of Education;

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