中国中药杂志

1997, (10)

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高效液相色谱法测定银杏叶提取物中槲皮素的含量
Highperformance Liquid Chromatographic Determination of Quercetin in Extract of Ginkgo biloba L. Leaves

周欣,钟世江,陈树琳
Zhou Xin,Zhong Shijiang and Chen Shulin (Physical and Chemical Analysis Research Center,Guizhou Industrial University,Guiyang 550003)

摘要(Abstract):

采用反相高效液相色谱法分离银杏叶提取物中黄酮类化合物并测定其中槲皮素含量。YWGC18(046cm×15cm)色谱柱,流动相甲醇水磷酸(50∶498∶02),检测波长360nm。回收率在953%~1032%之间,RSD为186%。
This paper reports the HPLC determination of one of the flavones quercetin in the extract of Ginkgo biloba leaves.Quercetin was separated completely on an YWG C 18 column(0.46cm×15cm),using a mixture of methanol,water and phosphoric acid(50∶49.8∶0.2) as mobile phase,with UV detection at 360nm.The results have shown the recoveries to be between 95.3%~103.2% and RSD 1.86%.

关键词(KeyWords): 高效液相色谱,槲皮素,银杏叶
highperformance liquid chromatography;quercetin;Ginkgo biloba leaves

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作者(Author): 周欣,钟世江,陈树琳
Zhou Xin,Zhong Shijiang and Chen Shulin (Physical and Chemical Analysis Research Center,Guizhou Industrial University,Guiyang 550003)

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