中国中药杂志

2008, (19) 2182-2184

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

双波长融合HPLC测定六味地黄丸中马钱苷、丹皮酚的含量
Determination of loganin and paeonol in Liuwei Dihuang pills by HPLC amalgamated of double UV waves

赵洪芝;孟宪生;叶挺祥;刘征辉;程奕;罗国安;
ZHAO Hong-zhi1,2,MENG Xian-sheng3,4,YE Ting-xiang1,2,LIU Zheng-hui1,2,CHENG Yi1,2,LUO Guo-an3(1.Tianjin High-standard Quality Testing Lab.,Tianjin 300084,China;2.Tianjin Center for Quality Testing of Traditional Chinese Medicine,Tianjin 300084,China;3.Department of Chemistry,Tsinghua University,Beijing 100084,China;4.Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

摘要(Abstract):

目的:建立同时测定六味地黄丸中的马钱苷和丹皮酚的含量的方法。方法:采用高效液相色谱法,Agilent Zorbax SB C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈-水线性梯度洗脱,柱温30℃,流速1.0 mL.min-1,检测波长236 nm(马钱苷),274 nm(丹皮酚);使用matlab编程进行谱图融合。结果:马钱苷与丹皮酚的线性范围分别为0.036 2~1.09(r=0.999 8);0.045 0~1.35μg(r=0.999 8);平均回收率分别为97.3%(RSD1.4%)和103.0%(RSD 1.9%)。结论:该方法为六味地黄丸的质控提供了更简便、合理、可靠的质控方法。
Objective: To establish a high performance liquid chromatographic(HPLC) amalgamated to double UV waves method for simultaneous determination of loganin and paeonol in Liuwei Dihuang pills.Method: A HPLC method was developed.The separation was carried out on a Agilent Zorbax SB C18 column(5 μm,4.6 mm×250 mm).The mobile phase consisted of water(A) and acetonitrile(B) with linear linear gradient elution [0-8 min,(B) from 1% to 12%;8-21 min,B keep 12%;21-40 min,(B) from 12% to 90%;40-50 min,B keep 90% for 10 min].The detection was Photodiode Array with the detection wavelengths were at 236 nm and 274 nm.The column temperature being 30 ℃ and the flow rate was 1.0 mL·min-1.Extracting the chromatergraph from 274 nm and 236 nm,we amalgamated the two chromatographs by matlab programmed.Result: The calibration curves of loganin and paeonol were linear in the ranges of 0.036 2-1.09 μg(r=0.999 8) and 0.045 0-1.35 μg(r=0.999 8),respectively.The average recoveries of loganin and paeonol were 97.3%(RSD 1.4 %) and 103.0%(RSD 1.9%),respectively.Three different batches of Liuwei Dihuang pills were determined with this method.Conclusion: This is a more convenient,reasonable and credible quality control method for the traditional Chinese medicine.

关键词(KeyWords): 六味地黄丸;高效液相色谱法;马钱苷;丹皮酚
Liuwei Dihuang pills;high performance liquid chromatography;loganin;paeonol

Abstract:

Keywords:

基金项目(Foundation): 天津市科技发展计划项目(05SYSYGX25000)

作者(Author): 赵洪芝;孟宪生;叶挺祥;刘征辉;程奕;罗国安;
ZHAO Hong-zhi1,2,MENG Xian-sheng3,4,YE Ting-xiang1,2,LIU Zheng-hui1,2,CHENG Yi1,2,LUO Guo-an3(1.Tianjin High-standard Quality Testing Lab.,Tianjin 300084,China;2.Tianjin Center for Quality Testing of Traditional Chinese Medicine,Tianjin 300084,China;3.Department of Chemistry,Tsinghua University,Beijing 100084,China;4.Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

Email:

DOI:

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享