中国中药杂志

2015, v.40(06) 1071-1074

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温度、密度、喂食周期对菲牛蛭育苗影响的研究
Effect of water temperature,stocking density and feeding cycle on growth of juvenile Poecilobdella manillensis

程搏幸;刘飞;郭巧生;史红专;李蒙蒙;卢昱希;
CHENG Bo-xing;LIU Fei;GUO Qiao-sheng;SHI Hong-zhuan;LI Meng-meng;LU Yu-xi;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;Guizhou Normal College;

摘要(Abstract):

研究了温度,密度,喂食周期对菲牛蛭幼苗的生长规律。分别设置7个温度(18,22,26,30,34,38℃和自然温度),5个密度(75,125,200,275,350条/L)和6个喂食周期(2,4,6,8,12,16 d),经30 d的养殖后考察各处理的存活率,增长率,特定增长率等生长指标。特定生长率与温度之间存在二次曲线相关,其回归方程式为y=-0.066x2+3.543 1x-38.09(R2=0.837 9),当水温为26.84℃,菲牛蛭幼苗的特定增长率为最大9.461 4,菲牛蛭幼苗最适生长水温在26~30℃。水温为38℃时,菲牛蛭幼苗全部死亡;特定增长率与密度之间存在显著直线回归关系,其回归方程式为y=-0.005 7x+9.197 3(R2=0.998 3);特定增长率与喂食周期之间存在显著直线回归关系,其回归方程式为y=-0.468 2x+10.574(R2=0.998 8)。
The effect of water temperature,stocking density and feeding cycle on the growth of Poecilobdella manillensis juvenile was conducted P. manillensis was conducted respectively under different conditions: water temperatures( 18,22,26,30,34,38 ℃and CT),stocking density( 75,125,200,275,350 individual / L) and feeding cycle( 2,4,6,8,12,16 d). After 30 days,survival rate,weight gain rate,specific growth rate were measured. There was a significant correlation between water temperature and specific growth rate( y =-0. 066x2+ 3. 543 1x-38. 09,R2= 0. 837 9). Based on the regression equation,the specific growth rate of P. manillensis achieved the maximum( 9. 461 4) at 26. 84 ℃. And the most optimal water temperature was 26-30 ℃. Meanwhile,the survival rates of P. manillensis was 0 at 38 ℃ in 3 d. There was significant negative correlation between density and specific growth rate( y =-0. 005 7x + 9. 197 3,R2= 0. 998 3) and between feeding cycle and specific growth rate( y =-0. 468 2x + 10. 574,R2= 0. 9988).

关键词(KeyWords): 菲牛蛭;幼苗;温度;密度;喂食周期;特定生长率
Poecilobdella manillensis;juvenile;temperature;stocking density;feeding cycle;specific growth rate

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基金项目(Foundation): 国家自然科学基金项目(81473306);; 中国博士后科学基金面上项目(2014M551614);; 江苏省博士后科研计划项目(1302032C)

作者(Author): 程搏幸;刘飞;郭巧生;史红专;李蒙蒙;卢昱希;
CHENG Bo-xing;LIU Fei;GUO Qiao-sheng;SHI Hong-zhuan;LI Meng-meng;LU Yu-xi;Institute of Chinese Medicinal Materials,Nanjing Agricultural University;Guizhou Normal College;

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