中国中药杂志

2017, v.42(14) 2738-2743

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

黄芪丹参水煎液抑制ISO诱导的大鼠心肌重构及其下调STIM1,TRPC1,CaN和NFATc3表达的机制
Huangqi Danshen decoction attenuates isoproterenol-induced myocardial remodeling via STIM1,TRPC1,CaN and NFATc3 pathways in rats

王新东;祁晓霞;卞勇;陈晓栋;张一炎;方祝元;
WANG Xin-dong;QI Xiao-xia;BIAN Yong;CHEN Xiao-dong;ZHANG Yi-yan;FANG Zhu-yuan;The First Clinical Medical College,Nanjing University of Chinese Medicine;Jiangsu Province Academy of Traditional Chinese Medicine;

摘要(Abstract):

探讨黄芪丹参水煎液(HDD)对异丙肾上腺素(ISO)诱导大鼠心肌重构抑制作用和对STIM1,TRPC1,Ca M,Ca N,NFATc3表达的影响。皮下注射ISO(2.5 mg·kg(-1)·d(-1)·d(-1),14 d)建立大鼠心肌重构模型,随机分为对照组、ISO模型组、HDD5(HDD 5 g·kg(-1),14 d)建立大鼠心肌重构模型,随机分为对照组、ISO模型组、HDD5(HDD 5 g·kg(-1)·d(-1)·d(-1)+ISO)组、HDD10(HDD 10 mg·kg(-1)+ISO)组、HDD10(HDD 10 mg·kg(-1)·d(-1)·d(-1)+ISO)组。干预4周后,计算心脏质量指数(HW/BW)和左心室质量指数(LVW/BW);超声心动图观察心肌结构;HE染色观察心肌组织病理学结构变化;ELISA检测血清中BNP,Ca N和Ca M kinasesⅡ的水平;Western blot检测左心室组织STIM1,TRPC1,p-Ca N,p-NFATc3和NFATc3蛋白的表达。结果显示,ISO组大鼠HW/BW和LVW/BW均大于HDD5组和HDD10组(P<0.05);超声心动图检查结果显示,HDD能够抑制ISO诱导的LVEDD,LVESD增加;ELISA检测显示,HDD能明显抑制ISO致心肌重构大鼠血清中BNP,Ca N和Ca M kinasesⅡ含量的升高(P<0.01)。Western blot检测结果显示,ISO组大鼠心肌组织STIM1,TRPC1,p-Ca N,p-NFATc3和NFATc3表达升高,HDD给药后心肌组织内STIM1,TRPC1,p-Ca N,p-NFATc3和NFATc3的表达均降低(P<0.05)。结果表明,黄芪丹参水煎液具有抑制ISO致大鼠心肌重构的作用,其机制与下调STIM1,TRPC1,Ca M kinasesⅡ,p-Ca N/Ca N和pNFATc3/NFATc3表达有关。
To investigate the inhibitory effect of Huangqi Danshen decoction( HDD) on isoproterenol( ISO)-induced myocardial remodeling and explore its effect on STIM1,TRPC1,Ca N and NFATc3 expressions. ISO( 2. 5 mg·kg(-1)+ISO)组。干预4周后,计算心脏质量指数(HW/BW)和左心室质量指数(LVW/BW);超声心动图观察心肌结构;HE染色观察心肌组织病理学结构变化;ELISA检测血清中BNP,Ca N和Ca M kinasesⅡ的水平;Western blot检测左心室组织STIM1,TRPC1,p-Ca N,p-NFATc3和NFATc3蛋白的表达。结果显示,ISO组大鼠HW/BW和LVW/BW均大于HDD5组和HDD10组(P<0.05);超声心动图检查结果显示,HDD能够抑制ISO诱导的LVEDD,LVESD增加;ELISA检测显示,HDD能明显抑制ISO致心肌重构大鼠血清中BNP,Ca N和Ca M kinasesⅡ含量的升高(P<0.01)。Western blot检测结果显示,ISO组大鼠心肌组织STIM1,TRPC1,p-Ca N,p-NFATc3和NFATc3表达升高,HDD给药后心肌组织内STIM1,TRPC1,p-Ca N,p-NFATc3和NFATc3的表达均降低(P<0.05)。结果表明,黄芪丹参水煎液具有抑制ISO致大鼠心肌重构的作用,其机制与下调STIM1,TRPC1,Ca M kinasesⅡ,p-Ca N/Ca N和pNFATc3/NFATc3表达有关。
To investigate the inhibitory effect of Huangqi Danshen decoction( HDD) on isoproterenol( ISO)-induced myocardial remodeling and explore its effect on STIM1,TRPC1,Ca N and NFATc3 expressions. ISO( 2. 5 mg·kg(-1)·d(-1)·d(-1)× 14 d) was given by subcutaneous injection to establish myocardial remodeling models in rats,and then were randomly divided into control group,ISO model group,HDD5group( HDD 5 g·kg(-1)× 14 d) was given by subcutaneous injection to establish myocardial remodeling models in rats,and then were randomly divided into control group,ISO model group,HDD5group( HDD 5 g·kg(-1)·d(-1)·d(-1)+ ISO),and HDD_(10) group( HDD 10 g·kg(-1)+ ISO),and HDD_(10) group( HDD 10 g·kg(-1)·d(-1)·d(-1)+ ISO). After intervention for 4weeks,the heart mass index( HW/BW) and the left ventricular mass index( LVW/BW) were calculated; the structure of myocardium was observed by echocardiography; the pathological changes of myocardium were observed by HE staining; levels of BNP,Ca N and Ca M kinases II in serum were detected by ELISA,and the protein expression levels of STIM1,TRPC1,p-Ca N,p-NFATc3,and NFATc3 in left ventricular tissues were detected by Western blot. The results showed that the HW/BW and LVW/BW in ISO group were greater than those in HDD5 group and HDD10group( P < 0. 05); Echocardiography showed that HDD inhibited ISO-induced increase in LVEDD and LVESD; ELISA results showed that HDD could significantly inhibit the increase of BNP,Ca N and Ca M kinases II levels in serum of rats with ISO-induced myocardial remodeling( P < 0. 01). Western blot results showed that STIM1,TRPC1,p-Ca N,p-NFATc3 and NFATc3 expression levels were increased in the myocardial tissues of ISO group rats,and after HDD administration,the above expression levels were decreased in group ISO,HDD for myocardial tissue after administration of STIM1,TRPC1,pCa N,p-NFATc3 and NFATc3 expression decreased( P < 0. 05). Our findings indicated that HDD can attenuate the myocardial remodeling induced by ISO,and its mechanism may be related to down-regulating the expression levels of STIM1,TRPC1,Ca M kinases II,p-Ca N/Ca N and p-NFATc3/NFATc3.

关键词(KeyWords): 黄芪丹参水煎液;心肌重构;基质相互作用分子1;瞬时受体电位通道1
Huangqi Danshen decoction;myocardial remodeling;STIM1;TRPC1

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金项目(81403386);; 江苏省自然科学基金青年基金项目(BK20161078);; 中国中医科学院江苏分院课题(JSBY1316)

作者(Authors): 王新东;祁晓霞;卞勇;陈晓栋;张一炎;方祝元;
WANG Xin-dong;QI Xiao-xia;BIAN Yong;CHEN Xiao-dong;ZHANG Yi-yan;FANG Zhu-yuan;The First Clinical Medical College,Nanjing University of Chinese Medicine;Jiangsu Province Academy of Traditional Chinese Medicine;

DOI: 10.19540/j.cnki.cjcmm.20170609.013

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享