中国中药杂志

2016, v.41(04) 716-721

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桃红四物汤各分离部位对体外ADP诱导血小板聚集、凝血酶活性的影响及效应物质基础研究
Effect of different fractions of Taohong Siwu decoction on ADP-induced platelet aggregation and thrombin activity

刘立;段金廒;宿树兰;刘培;唐于平;钱大玮;
LIU Li;DUAN Jin-ao;SU Shu-lan;LIU Pei;TANG Yu-ping;QIAN Da-wei;School of Pharmacy,Guangdong Medical College;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae Research,Nanjing University of Chinese Medicine;

摘要(Abstract):

评价桃红四物汤各分离部位对体外二磷酸腺苷(ADP)诱导血小板聚集及凝血酶活性的影响,探讨其效应物质基础。采用比浊法测定体外ADP诱导家兔血小板聚集率;采用改进凝血酶时间法测定抑制凝血酶活性;采用UPLC-Q-TOF-MS对各分离部位进行定性分析鉴定。桃红四物汤醇沉沉淀部位、醇沉上清液部位及20%~30%醇洗脱部位对ADP诱导的血小板聚集具有显著的抑制作用;水不溶部位、醇沉上清液部位及40%~70%醇洗脱部位具有显著的抑制凝血酶活性的作用;芳香酸类成分、苷类成分和苯酞类成分是醇沉沉淀部位、醇沉上清液部位及20%~40%醇洗脱部位的主要成分。桃红四物汤抑制ADP诱导家兔血小板聚集及抑制凝血酶活性的主要效应物质基础是芳香酸类成分、苷类成分和苯酞类成分。该实验为进一步探讨活血化瘀方桃红四物汤治疗血瘀证的物质基础及作用机制提供了科学依据。
To evaluate the effect of different fractions of Taohong Siwu decoction on ADP-induced platelet aggregation and thrombin activity,and to exploit the bioactive constituents,ADP-induced platelet aggregation rate in rabbits was determined by using the method of turbidity method. A bioassay called thrombin time was developed for determining anti-thrombin activities. UHPLC-Q-TOF-MS method was used to qualitatively analyze the chemical constituents of different parts. Alcohol precipitation deposition fraction,alcohol precipitation supernatant fraction and 20% to 30% alcohol elution fraction could significantly inhibit ADP-induced platelet aggregation.Alcohol precipitation supernatant fraction,water insoluble fraction and 40% to 70% alcohol elution fraction could significantly inhibit thrombin activity. The main components of alcohol precipitation deposition fraction,alcohol precipitation supernatant fraction and 20%to 40% alcohol elution fraction were analyzed and identified as aromatic acids,glycosides and phthalides. The bioactive constituents of Taohong Siwu decoction for inhibiting ADP-induced platelet aggregation and thrombin activity include aromatic acids,glycosides and phthalides. This experiment provides scientific basis to further explore the bioactive constituents and mechanism of Taohong Siwu decoction in treating blood stasis syndrome.

关键词(KeyWords): 桃红四物汤;血小板聚集;凝血酶活性;物质基础
Taohong Siwu decoction;platelet aggregation;thrombin activity;bioactive constituents

Abstract:

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基金项目(Foundation): 国家自然科学基金青年基金项目(81503465);; 广东省自然科学基金项目(2015A030310263);; 广东省中医药管理局项目(20131258);; 广东医学院博士启动项目(b2012007);; 江苏省高校自然科学重大基础研究项目(06KJA36022,10KJA360039);; 国家科技支撑计划项目(2008BAI51B01)

作者(Author): 刘立;段金廒;宿树兰;刘培;唐于平;钱大玮;
LIU Li;DUAN Jin-ao;SU Shu-lan;LIU Pei;TANG Yu-ping;QIAN Da-wei;School of Pharmacy,Guangdong Medical College;Jiangsu Key Laboratory for High Technology Research of Traditional Chinese Medicine Formulae Research,Nanjing University of Chinese Medicine;

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