中国中药杂志

2012, v.37(16) 2435-2438

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肾虚衰老模型的建立及其与D-半乳糖衰老模型的比较研究
Study on establishment of kidney deficient aging model and comparison with D-galactose induced aging model

李崭;刘仁慧;康学;王秀娟;
LI Zhan,LIU Renhui,KANG Xue,WANG Xiujuan(Department of Materia Medica,School of Traditional Chinese Medicine,Capital Medicine University,Beijing 100069,China)

摘要(Abstract):

目的:建立肾虚衰老模型,从抗氧化能力,HPAT(下丘脑-垂体-肾上腺-胸腺)轴功能,骨代谢等方面对其进行评价,并与D-半乳糖衰老模型进行比较。方法:皮下注射D-半乳糖溶液模拟大鼠衰老模型,同时腹腔注射地塞米松溶液建立肾虚衰老模型,以血清丙二醛(MDA)含量,谷胱甘肽过氧化物酶(GSH-Px)活力及肝脏超氧化物歧化酶(SOD)活力,肾上腺、胸腺及脾脏脏器指数,外周全血CD4+,CD8+,血清皮质醇(COR),骨钙素(BGP)及血浆促肾上腺皮质激素(ACTH),促肾上腺皮质激素释放激素(CRH)含量为指标,对模型进行评测。结果:与正常对照组比,衰老模型组、肾虚衰老模型组肝脏SOD活力极显著降低(均P<0.01),血清MDA含量极显著升高(均P<0.01),肾虚衰老模型组血浆ACTH含量显著升高(P<0.05)。与对照组和衰老模型组比,肾虚衰老模型组大鼠体重极显著降低(均P<0.01),血清GSH-Px活力表现为极显著降低和显著降低(P<0.01,P<0.05),肾上腺指数表现为显著降低和极显著降低(P<0.05,P<0.01),血清COR含量显著降低(均P<0.05),血浆CRH含量表现为显著升高和极显著升高(P<0.05,P<0.01),血清BGP含量极显著降低(均P<0.01),CD4+显著降低,CD8+极显著降低(P<0.05,P<0.01),CD4+/CD8+升高,但无显著性差异。结论:肾虚衰老模型抗氧化能力降低,HPAT轴功能紊乱,骨代谢异常。而D-半乳糖衰老模型仅在抗氧化能力方面表现出明显的差异。
Objective: To establish a kidney deficient aging model(KDAM),assess it in antioxidant capacity,HPAT axis function and bone metabolism,and compare with D-galactose aging model.Method: Aging rat model was established by injecting D-galactose solution,meanwhile dexamethasone solution was injected to establish kidney deficient aging model.Then these models were evaluated by serum MDA(malondialdehyde) and GSH-Px(glutathione peroxidase),liver SOD(superoxide dismutase),adrenal,thymus and spleen index,CD4+,CD8+,and serum COR(cortisol),BGP(bone Gla-protein),plasma ACTH(adrenocorticotropic hormone) and CRH(corticotropin-releasing hormone).Result: Compared with the normal group,the aging model group and the kidney deficient aging group showed significant decrease in liver SOD activity(P<0.01 on average) and significant increase in serum MDA content(P<0.01 on average),and the kidney deficient aging group revealed remarkable decline in plasma ACTH content(P<0.05).Compared with the normal group and the aging model group,the kidney deficient aging model group′s weight,serum GSH-Px decreased(P<0.01,P<0.05),adrenal index decreased(P<0.05,P<0.01),serum COR decreased(P<0.05 on average),plasma CRH increased(P<0.05,P<0.01),serum BGP content significantly decreased(P<0.01 on average),value of CD4+,CD8+ decreased(P<0.05,P<0.01),CD4+/CD8+ increased,but without significant difference.Conclusion: The kidney deficient aging model shows significant decrease in antioxidant capacity,dysfunction of HPAT axis disorder and abnormal bone metabolism.However,D-galactose aging model only shows a significant difference in antioxidant capacity.

关键词(KeyWords): 衰老模型;肾虚;氧化损伤;HPAT轴
aging model;kidney deficiency;oxidative damage;HPAT axis

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基金项目(Foundation): 首都中医药与护理研究专项课题(10ZYH03)

作者(Author): 李崭;刘仁慧;康学;王秀娟;
LI Zhan,LIU Renhui,KANG Xue,WANG Xiujuan(Department of Materia Medica,School of Traditional Chinese Medicine,Capital Medicine University,Beijing 100069,China)

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