中国中药杂志

2009, v.34(09) 1121-1123

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HPLC同时测定前胡中3种香豆素成分的含量
Simultaneous determination of three coumarin constituents in roots of Peucedanum praeruptorum by RP-HPLC

吴文玲;陈佳佳;刘守金;俞年军;方成武;张庆庆;
WU Wenling,CHEN Jiajia,LIU Shoujin,YU Nianjun,FANG Chengwu,ZHANG Qingqing(Anhui Key Laboratory of Modernized Chinese Material,Anhui University of Traditional Chinese Medicine,Hefei 230031,China)

摘要(Abstract):

目的:建立了用HPLC法对前胡药材中白花前胡甲素、乙素和E素同时定量的方法。方法:高效液相色谱法,色谱柱Agilent TC-C18(4.6 mm×250 mm,5μm);流动相甲醇-水(75∶25);检测波长321 nm;柱温30℃;流速0.8 mL.min-1;进样量20μL。结果:白花前胡甲素、乙素和E素的浓度与峰面积,分别在3.20~28.80μg(r=0.9999),1.60~14.40μg(r=0.9995),1.64~14.76μg(r=0.9994)呈良好的线性关系;平均加样回收率分别为98.92%,99.66%,99.72%,RSD分别为1.6%,1.5%,1.4%。结论:不同产地前胡所含的香豆素成分含量存在一定的差异,安徽宁国和祈门的前胡中白花前胡甲素含量高于药典的规定,且这3种香豆素含量明显高于其他2个产区。该法适合同时测定前胡样品中3种香豆素成分的含量,分离度好,快速,简便,重现性好。
Objective: To develop a reserved-phase HPLC method for the determination of praeruptorin A,praeruptorin B,qianhucoumarin E in roots of Peucedanum praeruptorum.Method: Agilent TC-C18 column(4.6 mm×250 mm,5 μm) was used at 30 ℃ with the mobile phase of methanol-water(75∶25).The flow rate was set at 0.8 mL·min-1.The detection wavelength was 321 nm.Result: The linear response ranged from 3.20-28.80 μg for ±praeruptorin A(r=0.999 9,n=5),1.60-14.40 g for praeruptorin B(r=0.999 5,n=5) and 1.64-14.76 g for qianhucoumarin E(r=0.999 4,n=5),respectively.Recoveries were 98.92% with RSD 1.6% for praeruptorin A,99.66% with RSD 1.5% for praeruptorin B and 99.72% with RSD 1.4% for qianhucoumarin E.Conclusion: The method is quick,simple and repeatable for determination of three coumarin constituents in root of P.praeruptorum.

关键词(KeyWords): 前胡;白花前胡甲素;白花前胡乙素;前胡E素;高效液相色谱法
Peucedanum praeruptorum;praeruptorin A;praeruptorin B;qianhucoumarin E;HPLC

Abstract:

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基金项目(Foundation): 安徽省教育厅自然基金重点课题(2006KJ096A);; 美国许氏基金会项目

作者(Author): 吴文玲;陈佳佳;刘守金;俞年军;方成武;张庆庆;
WU Wenling,CHEN Jiajia,LIU Shoujin,YU Nianjun,FANG Chengwu,ZHANG Qingqing(Anhui Key Laboratory of Modernized Chinese Material,Anhui University of Traditional Chinese Medicine,Hefei 230031,China)

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