中国中药杂志

2020, v.45(14) 3432-3440

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红花 CtWD40转录因子家族基因的生物信息学分析
Bioinformatics analysis of safflower WD40 transcription factor family genes

王刚;章正仁;王一非;洪瑛琦;刘秀明;姚娜;董园园;李海燕;
WANG Gang;ZHANG Zheng-ren;WANG Yi-fei;HONG Ying-qi;LIU Xiu-ming;YAO Na;DONG Yuan-yuan;LI Hai-yan;College of Life Science, Jilin Agricultural University;Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University;

摘要(Abstract):

WD40转录因子家族是真核生物中广泛存在的基因超家族,与植物生长及发育调控密切相关。并且在以往的研究中发现WD40转录因子参与花色苷的合成,而花色苷是红花类黄酮化合物的重要组成之一。该研究通过生物信息学手段及基因表达分析方法鉴定了红花基因组中40个CtWD40家族成员。研究根据拟南芥等植物WD40蛋白序列及系统发育特点将红花CtWD40家族归为7个亚家族,结合保守基序分析揭示每个亚家族成员特定的保守基序与基因结构,发现进化关系邻近的家族成员在保守基序与基因结构上具有一定的相似性。其次,基因启动子顺式作用元件搜索发现CtWD40特定启动子元件,揭示其可能参与的代谢途径。接下来,使用功能富集分析,分析CtWD40蛋白的功能类别和细胞定位,并通过CtWD40蛋白间相互作用,预测其潜在的调控功能。最后,根据qRT-PCR揭示了红花CtWD40亚家族19个成员在红花不同组织及花期中的差异表达。该研究为红花CtWD40基因功能验证及表达分析提供了理论依据。
The WD40 transcription factor family is a gene superfamily widely found in eukaryotes, which is closely related to plant growth and development regulation. It has been reported that the WD40 transcription factor was involved in the synthesis of anthocyanins, which is one of the vital components of safflower flavonoid compounds. In this study, 40 CtWD40 members in the safflower genome were identified though bioinformatics tools and gene expression analysis methods. According to the WD40 protein sequence and phylogenetic characteristics of Arabidopsis and other plants, the safflower CtWD40 family was classified into 7 subfamilies. Conservative motif analysis was used to reveal the specific conserved motifs and gene structures of each subfamily member, and there exist a certain degree of similarities in the conserved motifs and gene structure between the closely related family members. Subsequently, the search for cis-acting elements of gene promoters found CtWD40-specific promoter elements, revealing the metabolic pathways which may involve. Next, enrichment of function analysis was employed to analyze the functional categories and cellular localization of the CtWD40 protein. Furthermore, the interactions between CtWD40 proteins predicted its potential regulatory function. Finally, 19 members of the safflower CtWD40 subfamily were analyzed by qRT-PCR, the result showed the expression patterns of these members were different in diverse tissue and flowering period. This study provides a basis for the functional and expression research of the CtWD40 genes.

关键词(KeyWords): 红花;WD40基因;生物信息分析;表达分析;系统发育
safflower;WD40 gene;bioinformatics analysis;expression analyses;phylogeny

Abstract:

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基金项目(Foundation): 国家自然科学基金项目(31771868);; 吉林省科技厅自然科学基金项目(20190201172JC,20190201175JC);; 吉林省教育厅科学研究规划项目(JJKH20190918KJ)

作者(Author): 王刚;章正仁;王一非;洪瑛琦;刘秀明;姚娜;董园园;李海燕;
WANG Gang;ZHANG Zheng-ren;WANG Yi-fei;HONG Ying-qi;LIU Xiu-ming;YAO Na;DONG Yuan-yuan;LI Hai-yan;College of Life Science, Jilin Agricultural University;Bioreactor and Drug Development, Ministry of Education, Jilin Agricultural University;

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