中国中药杂志

2005, (07) 526-530

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参麦注射液对大鼠脑出血后迟发性神经细胞损伤的保护作用
Protective effects of Shenmai injection on the delayed injury of the cerebral neurons in rat induced by intracerebral hemorrhage

何泽云,李晓峰,屈波
HE Ze-yun, LI Xiao-feng, QU Bo(1. The Postdoctorate Research Centre of The First Affiliated Hospital, Zhejiang University, Hangzhou 310003,China; 2. Hunan College of Traditional Chinese Medicine ,Changsha 410007,China)

摘要(Abstract):

目的:观察参麦注射液对大鼠脑出血后迟发性神经细胞损伤的保护作用。方法:采用Rosenberg法复制大鼠脑出血模型,观察脑出血后大鼠海马CA1区神经细胞病理形态结构、神经细胞线粒体DNA缺失、C-myc基因、PDGFA基因表达及参麦注射液的防治作用。结果:参麦注射液减少大鼠脑出血后海马CA1区锥体细胞凋亡数目,抑制血肿周围脑组织线粒体DNA片段缺失,参麦注射液对出血早期C-mycmRNA的表达无明显影响,但可使PDGFAmRNA表达明显下调并时限延长。结论:参麦注射液可能通过调节PDGF表达对脑出血后迟发性神经细胞损伤修复有保护作用。
Objective: To observe protective effects of Shenmai (SM)injection on the delayed injury of the cerebral neurons in rat with intracerebral hemorrhage. Method: Rosenberg models of intracerebral hemorrhage was established and the effects of SM injection on the pathologic changes in neuronal structure, mitochondria-DNA(mtDNA)deletion, C-myc gene and expression PDGF-A gene in hippocampal CA 1 areas, were investigated. Result: SM injection inhibited the apoptosis of pyramidal cells in the hippocampal CA 1 areas, and decreased the degree of mtDNA deletion in the neurons in the injured area. SM injection had no effect on gene expression of C-myc at initial stage a intracerebral hemorrhage, but significantiy decreased the level of PDGF-A mRNA and prolonged the time of its expression. Conclusion: SM injection might attenuate the delayed injury induced by intracerebral hemorrhage via regulating the expression of PDGF.

关键词(KeyWords): 参麦注射液;脑出血;线粒体DNA;PDGF基因
Shenmai injection; intracerebral; hemorrhage; mitochondria-DNA; PDGF gene

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(39900199);; 湖南省自然科学基金(99JJY2003);; 中国博士后基金(2003034521)

作者(Authors): 何泽云,李晓峰,屈波
HE Ze-yun, LI Xiao-feng, QU Bo(1. The Postdoctorate Research Centre of The First Affiliated Hospital, Zhejiang University, Hangzhou 310003,China; 2. Hunan College of Traditional Chinese Medicine ,Changsha 410007,China)

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