中国中药杂志

2018, v.43(22) 4513-4518

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

竹节参总皂苷通过调节TGF-β1/Smad3通路改善衰老大鼠心肌纤维化的作用研究
Effect of saponins extracted from Panax japonicas on inhibiting myocardial fibrosis by TGF-β1/Smad3 signaling pathway in aging rats

宋亚男;王烙佩;郑杰;张长城;王婷;袁丁;周志勇;
SONG Ya-nan;WANG Luo-pei;ZHENG Jie;ZHANG Chang-cheng;WANG Ting;YUAN Ding;ZHOU Zhi-yong;Medical College of Three Gorges University;Renhe Hospital of Three Gorges University;Yichang Key Laboratory of Ischemic Cardiovascular Disease Translational Medicine(Three Gorges University);

摘要(Abstract):

研究竹节参总皂苷(saponins extracted from Panax japonicas,SPJ)对衰老大鼠心肌纤维化的改善作用及作用机制。将18月龄SD雄性大鼠随机分为衰老模型组、SPJ低剂量组、SPJ高剂量组,每组10只;衰老模型组,SPJ低、高剂量组分别灌胃给予等量的生理盐水,SPJ 10,60 mg·kg-1·d-1,每周6次,持续灌胃6个月至24月龄;另取10只6月龄大鼠作为青年对照组。处死动物后,采用HE染色观察各组大鼠心肌形态; Masoon染色观察各组大鼠胶原沉积的变化; RT-PCR法检测各组大鼠心脏组织中胶原相关蛋白COL1α2,COL3α1,肌成纤维细胞标记物α-SMA和基质金属蛋白酶MMP9,MMP2的mRNA表达水平;Western blot法检测各组大鼠心脏组织中TGF-β1,p-Smad3,IL-1β,TNF-α的蛋白表达。SPJ能有效改善衰老大鼠心肌纤维紊乱程度,减少炎性细胞浸润,降低衰老大鼠胶原蛋白沉积;能明显下调衰老心脏组织中COL1α2,COL3α1,α-SMA和MMP9,MMP2的mRNA表达水平;同时,能有效抑制衰老心脏中TGF-β1,p-Smad3,TNF-α,IL-1β蛋白的表达量。SPJ能有效改善衰老大鼠心肌纤维化程度,其作用机制与抑制衰老心脏中TGF-β1,p-Smad3蛋白表达,降低心肌炎症反应有关。
To investigate the amelioration effect of saponins extracted from Panax japonicas(SPJ) on myocardial fibrosis in natural aging rats and its mechanisms,male SD rats aged 18 months were randomly divided into 3 groups(aging model group,low-dose SPJ group and high-dose SPJ group),with 10 rats in each group. SPJ groups were given SPJ at different doses(10,60 mg·kg-1·d-1)consecutively for 6 months,meanwhile,aging model group was treated with the equal volume of saline for 6 months until 24 months old. Another 10 rats aged 6 month were used as young control group. The changes of myocardial morphological were observed by haematoxylin-eosin(HE) staining. Masson staining was used to observe the changes of collagen deposition in rat hearts. RT-PCR was used to detect the mRNA expression levels of myofibroblast marker α-SMA,collagen-related protein COL1α2,COL3α1 and matrix metalloproteinase MMP2,MMP9. Western blot was used to test the changes of the protein expressions of TGF-β1,p-Smad3,IL-1β and TNF-α in heart tissues. SPJ can effectively improve the arrangement of myocardial fibers,decrease inflammatory infiltration and reduce collagen deposition in aging rats. SPJ can effectively down-regulate the mRNA expression levels of COL1α2,COL3α1,α-SMA,MMP9,MMP2 and inhibit the protein expressions of TGF-β1,p-Smad3,TNF-α,IL-1β in the natural aging heart tissues. SPJ can effectively alleviate myocardial fibrosis in natural aging rats,and its mechanisms was related to the inhibition of the protein expressions of TGF-β1,p-Smad3 and the reduction of myocardial inflammation in rat hearts.

关键词(KeyWords): 竹节参总皂苷;自然衰老大鼠;心肌纤维化;炎症反应;TGF-β1/Smad3信号通路
saponins extracted from Panax japonicas;natural aging rats;myocardial fibrosis;inflammation;TGF-β1/Smad3 signaling pathway

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31570329);; 缺血性心脑疾病转化医学重点实验室(三峡大学)开放基金项目(2016KXN05,2017KXN07)

作者(Author): 宋亚男;王烙佩;郑杰;张长城;王婷;袁丁;周志勇;
SONG Ya-nan;WANG Luo-pei;ZHENG Jie;ZHANG Chang-cheng;WANG Ting;YUAN Ding;ZHOU Zhi-yong;Medical College of Three Gorges University;Renhe Hospital of Three Gorges University;Yichang Key Laboratory of Ischemic Cardiovascular Disease Translational Medicine(Three Gorges University);

Email:

DOI: 10.19540/j.cnki.cjcmm.20180724.006

参考文献(References):

扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享