中国中药杂志

2008, (22) 2607-2611

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竹节参总皂苷的制备工艺及含量测定
Studies on preparative technology and quantitative determination for extracts of total saponin in roof of Panax japonicus

何毓敏;鲁科明;袁丁;张长城;
HE Yu-min,LU Ke-ming,YUAN Ding,ZHANG Chang-cheng(Medical Science College of China Three Gorges University,Yichang 443002,China)

摘要(Abstract):

目的:优选竹节参总皂苷的提取纯化工艺,并测定其含量。方法:运用正交试验设计,以药材中总皂苷的转移率作为考察指标,筛选最佳提取工艺;用正丁醇萃取合并醇溶-丙酮沉淀法纯化得到精制皂苷;分别采用分光光度法和HPLC测定其中总皂苷和三萜苷元齐墩果酸的含量。结果:提取工艺以药材粗粉10倍量的60%乙醇,浸泡2 h后加热回流提取3次,每次3 h最佳;纯化工艺中以正丁醇萃取3次,以85%乙醇充分溶解后,边搅拌边缓慢加入4~5倍量丙酮沉淀效果最佳。经纯化后总皂苷可达83.48%,其中三萜苷元齐墩果酸可达38.30%。结论:本工艺条件对竹节参总皂苷的提取收率高且稳定,测定方法简便可行,重现性好。
Objective: To explore the optimum extraction and purification condition of the total saponins in the root of Panax japonicus(RPJ),and establish its quality control methods.Method: Designed L16(45) orthogonal test with the extraction rate of total saponins as index,to determine the rational extraction process,and the techniques of water-saturated n-butanol extraction and acetone precipitation were applied to purify the alcohol extract of RPJ.Total saponins were detected by spectrophotometry and its triterpenoidal sapogenin oleanolic acid detected by HPLC.Result: The optimum conditions of total saponins from RPJ was as follows: the material was pulverized,dipped in 60% ethanol aqueous solution as extract solvent at 10 times of volume,and refluxed 3 times for 3 h each time.Extractant of water-saturated n-butanol with extraction times of 3 and precipitant of acetone with precipitation amount of 4-5 times were included in the purification process,which would obtain the quality products.The content of total saponins could reach to 83.48%,and oleanolic acid to 38.30%.Conclusion: The optimized preparative technology is stable,convenient and practical.The extract rate of RPJ was high and steady with this technology,which provided new evidence for industrializing production of the plant and developing new drug.

关键词(KeyWords): 竹节参;皂苷;齐墩果酸;制备工艺;含量测定
Panax japonicus;total saponins;oleanolic acid;preparative technology;content determination

Abstract:

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基金项目(Foundation): 湖北省自然科学基金项目(2006ABA204);; 湖北省科技攻关计划项目(2005AA301C06)

作者(Author): 何毓敏;鲁科明;袁丁;张长城;
HE Yu-min,LU Ke-ming,YUAN Ding,ZHANG Chang-cheng(Medical Science College of China Three Gorges University,Yichang 443002,China)

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