中国中药杂志

2015, v.40(11) 2180-2185

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开心散类方对慢性应激大鼠行为学及中枢单胺类神经递质的影响
Effects of Kaixin San formulas on behavioristics and central monoamine neurotransmitters of chronic stress rats

刘婉婉;许璐;董宪喆;谭潇;王石;朱维煜;刘屏;
LIU Wan-wan;XU Lu;DONG Xian-zhe;TAN Xiao;WANG Shi;ZHU Wei-yu;LIU Ping;Department of Clinical Pharmacology and Pharmacy,Chinese PLA General Hospital;Tianjin University of Traditional Chinese Medicine;Shanxi University of Traditional of Chinese Medicine;

摘要(Abstract):

开心散类方治疗抑郁症的疗效已得到多项研究证实,为研究组方相同、配比不同的6个开心散类方对慢性应激大鼠大脑单胺类神经递质的影响,并结合类方的入血成分差异,比较其抗抑郁作用,探讨药效-配伍的关系及可能的药效机制。利用经典孤养结合慢性不可预知性温和应激(CUMS)抑郁模型,并建立HPLC和UPLC-Q-TOF-MSE检测方法,研究大鼠海马和皮层单胺类神经递质(NE,DA,5-HT)的含量变化和部分类方的入血成分。结果显示,《备急千金要方》之定志小丸显著增加了模型大鼠的行为学得分,并且明显增加了模型大鼠海马NE,5-HT的含量以及皮层NE,DA,5-HT的含量,抗抑郁作用最显著;《古今录验》之定志丸明显增加了模型大鼠的糖水偏嗜度和旷场得分,显著增加了模型大鼠海马NE的含量以及皮层NE,DA,5-HT的含量,显示了一定的抗抑郁作用;《医心方》之开心散显著增加了模型大鼠的体重,明显增加了模型大鼠海马NE和皮层DA的含量;《千金翼》之补心汤明显增加了模型大鼠海马5-HT和皮层DA的含量;《医门方》之开心丸只显著增加了模型大鼠皮层DA的含量;《千金要方》之开心散未见抗抑郁作用。结果表明,类方抗抑郁作用的不同可能与入血成分的差异有关。
The efficacy of Chinese herbal formula in treating depression has been proved in many studies. In this study,six different Kaixin San formulas were compared to investigate their effects on central monoamine neurotransmitters of chronic stress rats and against depression based on their different components in plasma,in order to discuss the efficacy-comparability relationship and the possible efficacy mechanism. The classic isolation method and the chronic unpredictable mild stress( CUMS) depression model were combined to investigate the changes in contents in hippocampus and monoamine neurotransmitters( NE,DA,5-HT) and the components of some formulas in plasma with HPLC and UPLC-Q-TOF-MSE methods. As a result,Dingzhi Xiaowan recorded in Essential Recipes for Emergent Use Worth A Thousand significantly increased the behavioral scores,NE and 5-HT contents in hippocampus and NE,DA and5-HT contents in cortex,with the best anti-depressant effect. Dingzhi Xiaowan recorded in Complete Records of Ancient and Modern Medical Works showed a notable increase in sucrose preference and open field score in model rats,NE content in hippocampus and NE,DA and 5-HT contents in cortex,with a certain anti anti-depressant effect. Kaixin San recorded in Ishinpo showed remarkable rise in weight of model rats,NE content in hippocampus and DA content in cortex. Puxin Decoction recorded in A Supplement to Recipes Worth A Thousand Gold showed 5-HT content in hippocampus and DA content in cortex. Kaixin San recorded in Yimenfang only showed DA content in cortex. Kaixin Wan recorded in Essential Recipes for Emergent Use Worth A Thousand did not mention the antidepressant effect. According to the results,the formulas' different anti-depressant effects may be related to the different plasma components.

关键词(KeyWords): 开心散类方;抑郁症;CUMS;单胺类神经递质
Kaixin San formulas;depression;CUMS;single amine neurotransmitter

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81373996)

作者(Authors): 刘婉婉;许璐;董宪喆;谭潇;王石;朱维煜;刘屏;
LIU Wan-wan;XU Lu;DONG Xian-zhe;TAN Xiao;WANG Shi;ZHU Wei-yu;LIU Ping;Department of Clinical Pharmacology and Pharmacy,Chinese PLA General Hospital;Tianjin University of Traditional Chinese Medicine;Shanxi University of Traditional of Chinese Medicine;

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