王铁军;郭宏宝;卢迪;耿江;郭敏杰;郭绪林;
WANG Tie-jun,GUO Hong-bao,LU Di,GENG Jiang,GUO Min-jie,GUO Xu-lin (College of Pharmacy Peking University,100083 Beijing,China)

• 1:北京大学药学院
• 2:北京大学药学院
• 3:北京大学药学院
• 4:北京大学药学院

摘要(Abstract)：

目的:利用HPLC-ELSD测定不同产地商陆药材中商陆皂苷A的含量以控制其质量。方法:采用HPLC-ELSD含量测定法,Phenom enex Luna C18(4.6 mm×250 mm,5μm)色谱柱,以甲醇-水-冰醋酸(68∶31∶1)为流动相。结果:商陆皂苷A在4.05²0.24μg呈线性,回收率为98.8%,RSD 1.4%。测定11个不同来源的药材,其商陆皂苷A的含量在0.32%20.24μg呈线性,回收率为98.8%,RSD 1.4%。测定11个不同来源的药材,其商陆皂苷A的含量在0.32%¹.11%。结论:本法可快速、准确测定商陆药材中商陆皂苷A含量,可用于商陆药材的质量监控。
Objective: To develop a HPLC-ELSD method for the quantitation of esculentosides A in Radix Phytolacca.Method: A Phenomenex Luna C18 column(4.6 mm×250 mm,5 μm) with a solvent system consisting of methanol-water-acetic acid(68∶31∶1) was used,and detected by ELSD.Result: The calibration curve of esculentosides A showed good linearity in the range of 4.05-20.24 μg,the average recovery was 98.8%,with RSD of 1.4%.11 batches of Radix Phytolaccae were analyzed,and the contents were 0.32% to 1.11 %.Conclusion: The method is simple,rapid and sensitive,can be used for determination of esculentosides A in Radix phytolaccae.

关键词(KeyWords)： 商陆;商陆皂苷A;HPLC-ELSD
Phytolacca acinosa;HPLC-ELSD;esculentosides A

Abstract:

Keywords:

基金项目(Foundation): 国家重点科技攻关计划项目(99-929-01-25)

作者(Authors): 王铁军;郭宏宝;卢迪;耿江;郭敏杰;郭绪林;
WANG Tie-jun,GUO Hong-bao,LU Di,GENG Jiang,GUO Min-jie,GUO Xu-lin (College of Pharmacy Peking University,100083 Beijing,China)

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