王倩;许霞;张珂;张仕林;刘莉;伍庆;屠鹏飞;宋月林;赵云芳;李军;
WANG Qian;XU Xia;ZHANG Ke;ZHANG Shi-lin;LIU Li;WU Qing;TU Peng-fei;SONG Yue-lin;ZHAO Yun-fang;LI Jun;Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine;Guizhou Hanfang Pharmaceutical Co., Ltd.;Guizhou Key Laboratory of Mountainous Environment Information System and Environment Protection, Guizhou Normal University;

• 1:北京中医药大学中药学院中药现代研究中心
• 2:贵州汉方药业有限公司
• 3:贵州师范大学贵州省山地环境信息系统与生态环境保护重点实验室

摘要(Abstract)：

芪胶升白胶囊为苗药大品种，临床广泛用于多种癌症的辅助治疗。由黄芪、血人参等7味药物制成，其化学成分组尚未被阐明，质量控制水平较低。该研究采用LC-IT-TOF-MS全面采集芪胶的MS¹和MS1和MS²碎片信息，进而快速鉴定其化学成分。采用Capcell core ADME色谱柱(2.1 mm×150 mm, 2.7μm)进行分离，以0.1%甲酸水溶液(A)和乙腈(B)为流动相梯度洗脱；电喷雾质谱检测，正、负离子模式同时扫描获得高分辨质谱信息。通过推导质谱裂解规律、与对照品和文献数据比对等方式，共鉴定出107个化合物，包括54个黄酮类、16个苯酞类、13个生物碱类、12个酚酸类、7个皂苷类、2个香豆素类、2个缩合鞣质类和1个嘌呤类化合物。该研究深入阐明了芪胶升白胶囊化学成分组成，为其质量标准的提高及药效物质的阐明提供了丰富的信息。
Qijiao Shengbai Capsules(QJ) are a common Miao medicine serving as an adjuvant cancer therapy in clinical practice.QJ consists of seven medicinal materials such as Astragalus membranaceus and Lespedeza buergeri.Its chemical components have not been clarified and the quality control needs to be improved.In this study, LC-IT-TOF-MS was used to comprehensively collect MS2碎片信息，进而快速鉴定其化学成分。采用Capcell core ADME色谱柱(2.1 mm×150 mm, 2.7μm)进行分离，以0.1%甲酸水溶液(A)和乙腈(B)为流动相梯度洗脱；电喷雾质谱检测，正、负离子模式同时扫描获得高分辨质谱信息。通过推导质谱裂解规律、与对照品和文献数据比对等方式，共鉴定出107个化合物，包括54个黄酮类、16个苯酞类、13个生物碱类、12个酚酸类、7个皂苷类、2个香豆素类、2个缩合鞣质类和1个嘌呤类化合物。该研究深入阐明了芪胶升白胶囊化学成分组成，为其质量标准的提高及药效物质的阐明提供了丰富的信息。
Qijiao Shengbai Capsules(QJ) are a common Miao medicine serving as an adjuvant cancer therapy in clinical practice.QJ consists of seven medicinal materials such as Astragalus membranaceus and Lespedeza buergeri.Its chemical components have not been clarified and the quality control needs to be improved.In this study, LC-IT-TOF-MS was used to comprehensively collect MS¹ and MS1 and MS² fragment information of QJ and rapidly identify the chemical compositions.The chromatographic separation was performed on the Capcell core ADME column(2.1 mm×150 mm, 2.7 μm) with 0.1% formic acid aqueous solution(A) and acetonitrile(B) as mobile phases for gradient elution.High-resolution mass spectrometric information was obtained by scanning in the positive and negative ion ESI modes.A total of 107 compounds were structurally identified according to the deduced MS fragmentation patterns and comparison with standards and data reported in the literature, including 54 flavonoids, 16 phthalides, 13 alkaloids, 12 phenolic acids, 7 saponins, 2 coumarins, 2 condensed tannins, and 1 purine.This study clarified the chemical composition of QJ and provided references for the improvement of its quality standards and the elucidation of its medicinal substances.

关键词(KeyWords)： 化学成分分析;芪胶升白胶囊;UPLC-IT-TOF-MS;质量评价;黄酮
chemome profiling;Qijiao Shengbai Capsules;UPLC-IT-TOF-MS;quality evaluation;flavonoids

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划项目(2018YFC1707300)

作者(Authors): 王倩;许霞;张珂;张仕林;刘莉;伍庆;屠鹏飞;宋月林;赵云芳;李军;
WANG Qian;XU Xia;ZHANG Ke;ZHANG Shi-lin;LIU Li;WU Qing;TU Peng-fei;SONG Yue-lin;ZHAO Yun-fang;LI Jun;Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine;Guizhou Hanfang Pharmaceutical Co., Ltd.;Guizhou Key Laboratory of Mountainous Environment Information System and Environment Protection, Guizhou Normal University;

DOI: 10.19540/j.cnki.cjcmm.20220328.301

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