宋亚南;王云;高雅;张村;曲晓兰;杨洪军;
SONG Ya-nan;WANG Yun;GAO Ya;ZHANG Cun;QU Xiao-lan;YANG Hong-jun;Institute of Pharmacology, Shandong First Medical University;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;Experimental Research Center, China Academy of Chinese Medical Sciences;

• 1:山东第一医科大学药理学研究所
• 2:中国中医科学院中药研究所
• 3:中国中医科学院医学实验中心

摘要(Abstract)：

采用高效液相色谱法(HPLC)对辣木叶进行多成分(新绿原酸、L-色氨酸、维采宁-2、异槲皮素、紫云英苷)含量测定，绘制成分绝对含量-时间曲线，基于Fick扩散定律及希格比传质穿透模型理论，推算出方程参数，将测定结果代入数学模型进行方程拟合。结合时间因素和溶剂倍量因素下的方程所得到的n和a(两因素结果较为接近)，确立5个成分的煎煮动力学模型分别为■。基于多成分含量测定探究在不同煎煮时间及不同溶剂倍量下辣木叶煎煮的整体含量趋势，30 min及12倍量是与相邻参数具有(极)显著性差异的关键节点及最佳工艺参数。成分的溶出与破坏、成分的溶出扩散运动分别是造成辣木叶在不同煎煮时间及不同溶剂倍量下含量变化的关键所在。对辣木叶煎煮过程进行多成分含量监测，所建立的含量测定线性方程及煎煮过程线性方程(5～30 min及12～20倍量)的R²分别≥0.999 8及0.9，表明含量测定以及煎煮动力学模型的准确性高，因而能够较好地反映辣木叶的煎煮过程中的含量变化规律，为其煎煮工艺的优化提供参考。
Content of multiple components (neochlorogenic acid,L-tryptophan,vicenin-2,isoquercitrin,and astragalin) in Moringa oleifera leaves was determined by high-performance liquid chromatography (HPLC),and the absolute content-time curves were plotted.Based on Fick's law of diffusion and Higbie's penetration theory,the parameters of the equations were calculated,and the measured results were substituted into the mathematical model to fit the equations.The n and a obtained from the equations on the decocting time factor and the solvent volume were close to each other.The dynamic models of the five components are as follows:■.The variation of the content of multiple components in M.oleifera leaves with time and solvent volume was explored.It was found that the content of the components was the highest when the leaves were decocted for 30 min with solvent volume 12 folds of the medicinal material.The dissolution and destruction of components and the diffusion movement of components are the main causes of the content change of M.oleifera leaves at different time and with different solvent volumes.The R2分别≥0.999 8及0.9，表明含量测定以及煎煮动力学模型的准确性高，因而能够较好地反映辣木叶的煎煮过程中的含量变化规律，为其煎煮工艺的优化提供参考。
Content of multiple components (neochlorogenic acid,L-tryptophan,vicenin-2,isoquercitrin,and astragalin) in Moringa oleifera leaves was determined by high-performance liquid chromatography (HPLC),and the absolute content-time curves were plotted.Based on Fick's law of diffusion and Higbie's penetration theory,the parameters of the equations were calculated,and the measured results were substituted into the mathematical model to fit the equations.The n and a obtained from the equations on the decocting time factor and the solvent volume were close to each other.The dynamic models of the five components are as follows:■.The variation of the content of multiple components in M.oleifera leaves with time and solvent volume was explored.It was found that the content of the components was the highest when the leaves were decocted for 30 min with solvent volume 12 folds of the medicinal material.The dissolution and destruction of components and the diffusion movement of components are the main causes of the content change of M.oleifera leaves at different time and with different solvent volumes.The R²of the linear equations on the content and the equations on the decocting process (5-30min and solvent volume 12-20 folds of the medicinal materials) was≥0.999 8 and≥0.9,respectively.Thus,the content determination and the decocting kinetic model had high accuracy,which can reflect the change law of the content of key components in M.oleifera leaves during the decoction.This study is expected to serve as a reference for optimizing the decocting technology.

关键词(KeyWords)： 辣木叶;煎煮动力学模型;含量测定;高效液相色谱法(HPLC);水煎
Moringa oleifera leaves;decocting kinetic model;content determination;high-performance liquid chromatography;decoction with water

Abstract:

Keywords:

基金项目(Foundation): 云南省重大科技专项计划项目(2017ZF004)

作者(Authors): 宋亚南;王云;高雅;张村;曲晓兰;杨洪军;
SONG Ya-nan;WANG Yun;GAO Ya;ZHANG Cun;QU Xiao-lan;YANG Hong-jun;Institute of Pharmacology, Shandong First Medical University;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences;Experimental Research Center, China Academy of Chinese Medical Sciences;

DOI: 10.19540/j.cnki.cjcmm.20211112.301

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