高效液相色谱法同时测定大黄中14种成分的含量
投稿时间:2017-11-06  修订日期:2017-11-09  责任编辑:  点此下载全文
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作者中文名作者英文名单位中文名单位英文名E-Mail
刘月红 Liu Yuehong 北京中医药大学 Beijing University of Chinese Medicine liuyuehong@bucm.edu.cn 
黄政海 Huang Zhenghai 北京中医药大学 Beijing University of Chinese Medicine  
董玲 Dong Ling 北京中医药大学 Beijing University of Chinese Medicine  
裴纹萱 Pei Wenxuan 北京中医药大学 Beijing University of Chinese Medicine  
孙裕 Sun Yu 兰州佛慈制药股份有限公司 LANZHOU FOCI PHARMACEUTICAL CO., LTD  
高晓燕* Gao Xiaoyan 北京中医药大学 Beijing University of Chinese Medicine gaoxiaoyan0913@sina.com 
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
中文摘要:建立高效液相色谱法同时测定大黄中大黄素、大黄酚、大黄酸、芦荟大黄素、大黄素甲醚、番泻苷A、番泻苷B、没食子酸、儿茶素、(-)-表儿茶素-3-没食子酸酯、异莲花掌苷、4-4''-羟基苯基-2-丁酮、莲花掌苷、4''-羟基苯基-2-丁酮-4''-O-β-D-(2''-O-桂皮酰基-6''-O-没食子酰基)-葡萄糖苷14个成分含量的方法。采用Agilent Zorbax SB-C18色谱柱(4.6 × 150 mm, 5 μm),采用0.05%的磷酸水溶液(A)-乙腈(B)为流动相,梯度洗脱,流速1.0 mL/min,柱温40℃,检测波长268 nm。结果表明在线性范围内14个成分线性良好(r > 0.9999);日内精密度和日间精密度均小于3.1% ;平均回收率范围在91.8% ~ 104.1%。同时,对收集到的10个掌叶大黄合格样品和10个唐古特大黄合格样品进行定量测定,发现掌叶大黄中含量比较高的成分是芦荟大黄素,唐古特大黄中含量比较高的是4-4''-羟基苯基-2-丁酮,各样品中所有化合物的含量差异都比较大。所建立的含量测定方法能同时测定大黄中14个成分的含量,为大黄药材多成分含量测定和质量控制提供一种简便的方法。
中文关键词:大黄  高效液相色谱法  蒽醌类  苯丁酮苷类  鞣质类  含量测定
 
Simultaneous determination of 14 components in Rhei Radix et Rhizoma by high perfor-mance liquid chromatography method
Abstract:To establish an HPLC method for the simultaneous determination of gallic acid, (+)-catechin, (-)-epicatechin-3-O-gallate, isolindleyin, 4-(4''-hydroxyphenyl)-2-butanone, emodin, chrysophanol, physcion, aloe-emodin, rhein, lindleyin, 4-(4''-hydroxyphenyl)-2-butanone-4''-O-β-D-(2''-O-galloyl-6''-O-cinnamoyl)-glucopyranoside, sennoside A and sennoside B in Rhei Radix et Rhizoma. The analysis was performed on an Agilent Zorbax SB-C18 (4.6 × 150 mm, 5 μm) which 0.05% phosphoric acid (A) and ace-tonitrile (B) as mobile phase at flow rate of 1 mL/min in a gradient elution, and the wave-length was set at 268 nm. All calibration curves were show good linearity (r > 0.9999) within the concentration range. Both the intra- and inter-day precisions for 14 analytes were less than 3.1%, respectively, with the mean recovery at the range of 91.8% ~ 104.1%. Meanwhile, 14 components of 10 qualified samples from Rheum palmatum L. and 10 qualified samples from Rheum tanguticum Maxim. ex Balf. were quantitatively determined, respectively. It was found that the content of 4-(4''-hydroxyphenyl)-2-butanone and aloe-emodin and were higher in the Rheum tanguticum Maxim. ex Balf. and Rheum palmatum L., respectively. The content of all the compounds are different in each samples. The HPLC method for simultaneous determination of 14 contents of Rhei Radix et Rhizoma could be used for quantitative as-sessment and quality control of Rhei Radix et Rhizoma.
keywords:Rhei Radix et Rhizoma  HPLC  anthraquinones  butanone glycosides  tannins  content de-termination
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