Effects of salvianolic acid B on lipid peroxidation and metalloproteinase-2activity in fibrotic liver in rat
Received: September 11, 2009  
DOI:10.4268/cjcmm20100115
KeyWord:salvianolic acid B  liver cirrhosis  lipid peroxide  metalloproteinase  perindopril
AuthorInstitutionE-mail
WANG Lina Liver Disease Institute, Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Shanghai 201203, China;Department of Chinese Medicine, Second Military Medical University, Changhai Hospital, Shanghai 200433, China  
TAO Yanyan Liver Disease Institute, Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Shanghai 201203, China  
LI Shu Liver Disease Institute, Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Shanghai 201203, China  
CHEN Gaofeng Liver Disease Institute, Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Shanghai 201203, China  
LIU Chenghai Liver Disease Institute, Shanghai University of Traditional Chinese Medicine, Shuguang Hospital, Shanghai 201203, China;Experimental Center of Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China chenghai_liu@yahoo.com.cn 
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Abstract:
      Objective : To investigate the mechanism of salvianolic acid B (Sal B) action against liver fibrosis through preventing lipid peroxidation and regulating MMP-2 activity in liver. Method : The liver fibrotic model was induced through intraperitoneally injection of DMN at a dose of 10 μg·kg-1 for every other day and lasting for 4 weeks. Sal B was administered (10 mg·kg-1), and perindopril (5 mg·kg-1) was used as positive control. Hepatic inflammation and collagen were observed with HE and sirius red staining. The liver function including serum ALT, AST activity, Alb and total bilirubin (T.Bil) level were determined. The hepatic lipid peroxidation including SOD and GST activities and GSH content were measured. Hepatic hydroxyproline (Hyp) content was detected with Jamall's method. The activity of metalloproteinase was assayed by gelatin zymography. The expressions of α-SMA, Col I in liver tissue were analyzed by Western blot. Result : The model rats had higher serum T.Bil content, ALT and AST activities but lower Alb content than the normal rats, also had remarkable inflammatory necrosis and collagen deposition in liver, with much higher Hyp content, protein expression of α-SMA and collagen I and MMP-2 activity in liver, but had a decreased GSH content, SOD and GST activities. Both Sal B and perindopril attenuated hepatic injury and collagen deposition in model rats, decreased serum ALT activity and hepatic Hyp content, down-regulated α-SMA and collagen I protein expressions and metalloproteinase-2 activity than those in the model group, but increased SOD activity and GSH content, and Sal B decreased serum T.Bil content and increased GST activity. Sal B had a much better comprehensive actions than perindopril. Conclusion : Sal B has a good preventive action against liver fibrosis, the action mechanism is related to the prevention from lipid peroxidation and down-regulation of metalloproteinase-2 activity in fibrotic liver.
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