Polysaccharides from Scurrula parasitica L. inhibit sarcomaS180 growth in mice
Received: April 15, 2009  
DOI:10.4268/cjcmm20100328
KeyWord:Scurrula parasitica  polysaccharides  S180  immunohistochemistry
AuthorInstitutionE-mail
XIAO Yijun College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Fujian Key Laboratory of Developmeatal and Neuro Biology, Fuzhou 350108, China xiaoyijun@fjnu.edu.cn 
FAN Yanli College of Life Sciences, Fujian Normal University, Fuzhou 350108, China  
CHEN Binghua College of Life Sciences, Fujian Normal University, Fuzhou 350108, China  
ZHANG Qiujin College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;Fujian Key Laboratory of Developmeatal and Neuro Biology, Fuzhou 350108, China  
ZENG Hong College of Life Sciences, Fujian Normal University, Fuzhou 350108, China  
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Abstract:
      To study the anti-tumor activity of Scurrula parasitica polysaccharides(SP).Water extraction and ethanol precipitation were used to isolate SP from S. parasitica leaf.S180, K562 and HL-60 cell lines proliferation inhibition by SP were detected by MTT assay. The expressions of Ki-67, Cyclin D1, Bax and Bcl-2 protein in the sarcoma S180 tissues were detected by immunohistochemistry technique to approach the anti-tumor mechanism of SP+ SP could not inhibit cancer cell proliferation.SP ip could inhibit the growth of sarcoma S180 in mice, 100 mg·kg-1·d-1.SP ip was the optimal dose on inhibiting S180 growth, with the tumor inhibition rate of 54%.The expression of Ki-67, Cyclin D1, Bax and Bcl-2 protein in the sarcoma S180 tissues were detected by immunohistochemistry technique to approach the anti-tumor mechanism of SP.The result showed that SP could down-regulate the expression of Ki-67, CyclinD1 and Bcl-2 protein, and up-regulate the expression of Bax protein. It indicted that inhibiting cancer cell proliferation and promoting cancer cell apoptosis in vivo maybe one of the anti-cancer mechanisms of SP.
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