Effects of ABA and its biosynthetic inhibitor fluridone on accumulation of penolic acids and activity of PAL and TAT in hairy root of Salvia miltiorrhiza
Received: September 11, 2011  
DOI:10.4268/cjcmm20120614
KeyWord:Salvia miltiorrhiza  hairy root  ABA  phenolic acids
AuthorInstitutionE-mail
CUI Beimi College of Life Sciences, Northwest A& F University, Yangling 712100, China;Shaanxi Research Center in Traditional Chinese Medicine Fingerprint, Yangling 712100, China  
LIANG Zongsuo College of Life Sciences, Northwest A& F University, Yangling 712100, China;Shaanxi Research Center in Traditional Chinese Medicine Fingerprint, Yangling 712100, China liangzs@ms.iswc.cn 
LIU Yan Tianjin Tasly Modern Traditional Chinese Medicine Resources Co., Ltd., Tianjin 300410, China  
LIU Fenghua Tianjin Tasly Modern Traditional Chinese Medicine Resources Co., Ltd., Tianjin 300410, China  
ZHU Jianguo College of Life Sciences, Northwest A& F University, Yangling 712100, China  
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Abstract:
      Objective: To study the function of ABA and fluridone on the contents of penolic acids and two key synthetases (PAL and TAT). Method: Conducted 4 different concentrations in the hairy root of Salvia miltiorrhiza after culturing 18 days and treated with fluridone. One day later, harvested the hairy root and measured the activity of PAL and TAT; Treatment for 6 days, gathered and determined the contents of phenolic acids. Result: In certain concentration of ABA, lower ABA could induced the production of growth and higher ABA inhibitor the growth in hairy roots of S. miltiorrhiza; ABA induced the accumulation of caffeic acid considerably, and the effect on the contents of coffee acid show positive correlation; As for the RA and LAB, the low dosage of ABA simulated the production and higher ABA inhibited the production of them; the ABA biosynthetic inhibitor fluridone can decreases ABA's the effect; The different of ABA activated the activity of PAL and TAT, but the impact were discriminating, when treatment with ABA and fluridone, the inducing were declined. Conclusion: ABA induced the accumulation of
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