Regulatory effect of berberine on unbalanced expressions of renal tissue TGF-β1/SnoN and Smad signaling pathway in rats with early diabetic nephropathy
Received:March 08, 2012  
DOI:10.4268/cjcmm20122321
KeyWord:berberine  diabetic nephropathy  TGF-β1  SnoN  Smad signaling pathway
Author NameAffiliationE-mail
LIU Sheng Affiliated Provincial Hospital, Anhui Medical University, Hefei 230001, China lslcclhl@163.com 
YU Na Affiliated Provincial Hospital, Anhui Medical University, Hefei 230001, China;College of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230038, China  
ZHANG Xiao-li Affiliated Provincial Hospital, Anhui Medical University, Hefei 230001, China;College of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230038, China  
CHEN Xiang-qing Affiliated Provincial Hospital, Anhui Medical University, Hefei 230001, China  
TANG Li-qin Affiliated Provincial Hospital, Anhui Medical University, Hefei 230001, China  
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Abstract:
      Objective: To investigate the effect of berberine (BBR) on unbalanced expression of renal tissue TGF-β1/SnoN and Smad signal pathway in rats with early diabetic nephropathy (DN), and discuss BBR's effect on DN rats with early diabetic nephropathy and its possible mechanism. Method: DN rat model were established by injecting streptozotocin (STZ). The rats were divided into six groups: the control group, the model group, three BBR (50, 100, 200 mg·kg-1) treatment groups and the enalapril treatment group. They were orally administered once a day for five weeks. The fasting blood glucose (FBG), blood urea nitrogen (BUN), serum creatinine (Scr), urinary protein (24 h Upro) and urinary microalbumin (24 h UmAlb) were tested. The pathological changes in renal tissues were examined by optical microscopy. Immunohistochemical measures were used to detect the expressions of TGF-β1, SnoN, Smad2/3 and Smad7 protein, and RT-PCR was used to detect TGF-β1 mRNA in renal tissues. Result: Compared with the model group, BBR-treated groups showed significant decrease in FBG, BUN, Scr, 24 h Upro, 24 h UmAlb, TGF-β1 protein, mRNA and Smad2/3 protein, abnormal morphological improvement in renal tissues, and notable increase in the expressions of SnoN and Smad7 protein. Conclusion: BBR can maintain the dynamic balance in TGF-β1/SnoN expression in renal tissues through Smad signaling pathway, so as to mitigate renal functional disorder in DN rats and delay DN and its development.
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